A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo

分类： 生物学 >> 生物物理学 提交时间： 2016-05-11

Phiwpan, Krung Guo, Jie Zhang, Wei Hu, Tanyu Zhang, Jianhua Zhou, Xuyu Phiwpan, Krung Hu, Tanyu Boruah, Bhargavi M.

摘要： MicroRNA-155 (miR-155) plays significant role in various physiological processes involving both innate and adaptive immunity. miR-155 expression level changes dynamically during various immune responses. However, current approaches for miR-155 detection at the RNA level do not precisely reflect the real-time activity. Herein, we generated a transgenic mouse line (R26-DTR-155T) for determination of miR-155-5p activity in vivo by inserting miR-155-5p target sequence downstream of a reporter transgene comprising Diphtheria Toxin Receptor and TagBlue fluorescence protein. Using this approach, R26-DTR-155T mice were able to measure variation in levels of miR-155-5p activity in specific cell types of interest. The DTR expression levels were inversely correlated with the endogenous miR-155 expression pattern as detected by quantitative RT-PCR. Our data demonstrate a novel transgenic mouse line which could be useful for tracing miR-155-5p activity in specific cell types through measurement of miR-155-5p activity at single cell level.

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