Subjects: Biology >> Botany Subjects: Biology >> Biochemistry submitted time 2023-10-23
Abstract： The protein-protein interactions (PPIs) have a pivotal role in plant cells. There are numerous methods, in vivo or in vitro, have been developed to investigate the PPIs. The yeast mating-based Split-Ubiquitin System (mbSUS) is an alternative method of classic yeast-two-hybrid (Y2H) method. The mbSUS assay is not only as fast and inexpensive as the Y2H method, but also suitable for membrane proteins investigation with lower false positive rate. The recently improved mbSUS assay is suitable for the determination of PPIs among membrane proteins and soluble proteins, and successful used for detecting the interaction between ion channels and vesicle traffic proteins. Here, we describe the principles, protocols and experiment notes for mbSUS assay, providing an alternative method for PPI research in plants.
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