Subjects: Physics >> Interdisciplinary Physics and Related Areas of Science and Technology submitted time 2016-05-08
Abstract： Single-molecule experiments revealed that爐he爎eplication rate爋f燭7燚NA爌olymerase爃as燼爌eak value at爐ension爋f燼bout 5.5 pN. However,爐he爉echanism leading to this peak has only been partially investigated. Here, we present燼爐wo-state model to investigate爐he爀ffect爋f爉echanical爐ension爋n燚NApolymerase燼ctivity.燭he爉odel consists爋f爌olymerase燼nd exonuclease.燭he爌olymerase's kinetic pathway has been simplified into Michaelis-Menten formwith爐wo steps. One is爐he燾onformational change from "open" to "close".燭he爋ther is爐he燽ase-stacking.燭he爎esults are in good agreement爓ith爀xperimental observations. We also predict that爐he爐ension爋n爐emplate is beneficial to爐he爁idelity爋f燚NA爎eplication.
Peer Review Status:
Awaiting Review
Subjects: Physics >> Interdisciplinary Physics and Related Areas of Science and Technology submitted time 2016-05-08
Abstract： Within the framework of a two-state ratchet model, cooperative effects of collective molecular motors on the kinetics of adenosine triphosphate (ATP) hydrolysis have been studied in detail. We calculate the dependence of the rate of ATP consumption on the applied load. Features different from a single molecular motor have been found. We analyze two typical oscillations of collective molecular motors. The relations between the ATP concentration and oscillatory characteristics, such as frequency, amplitude, and the duty ratio, have been discussed.
Peer Review Status:Awaiting Review
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