Subjects: Biology >> Bioengineering submitted time 2017-09-20
Abstract： In this paper, the strain ZZ-46 which could assilimated glucose and xylose simultaneously producing lipid were screened from 10 wild yeast strains from the soil of Nanyang region of henan province. The cell biomass, lipid concentration, lipid content, lipid coefficient and lipid productivty of the strain reached 20.23g/L、9.89 g/L、48.91%、14.64g/100g and 0.083 g•L-1•h-1 respectively by fermenting 120h with 2: 1of glucose and xylose as carbon source. Simultaneous, Lipid-acid component was analyzed, there is almost no difference in lipid-acids component by using different mixed sugar ratios as carbon source and C16 and C18 series are main fatty acids. Oleic acid content is the highest in lipid-acid component, next are linoleic acid, palmitic acid and stearic acid in all samples and the sum account for over 90%. The lipid component of this strain is similar to plant oil and could be used as biodiesel source. The strain was primarily identified as Cutaneotrichosporon dermatis by the morphology feature and 26S rDNA D1/D2 domain sequence analysis, and Trichosporon dermatis homonym.
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