Subjects: Biology >> Botany >> Applied botany submitted time 2024-06-20 Cooperative journals: 《广西植物》
Abstract： The purpose of this study was to investigate the novelty and particularly of the culturable bacteria in seagrass beds and study its antibacterial activity. A total of nine rhizosphere soil samples were collected from the seagrass bed ecosystems in Pearl Bay and Yuzhouping, Fangchenggang City. Five different isolation medias were used to isolate and purify the culturable bacteria using the dilution spread plate method. The bacterial species were identified through PCR amplification and 16S rRNA sequencing. The antibacterial activity against four human pathogenic bacteria (Methicillin-resistant Staphylococcus aureus, S. epidermidis, S. aureu and Acinetobacter baumanii) were screened with Kirby-Bauer method. The results were as follows: (1)42 culturable bacteria were isolated, belonging to 14 genera and 13 families, which included Streptomyces, Vibrio, Bacillus, Shewanella, Microbacterium, Brevibacteriu, Staphylococcus, Sphingomona, Rothia, Psychrobacter, Pseudonocardia, Photobacterium, Halomonas, and Escherichia. The genus Streptomyces was the dominant bacterium. (2)Antibacterial activity showed that six bacterial exhibited strong inhibitory activity against at least two or more human pathogenic bacteria, which included Vibrio (1 bacteria), Rothia (1 bacteria), and Streptomyces (4 bacteria). The studies have shown that there are abundant resources of culturable bacterium and several bacteria has the potential antibacterial activity especially Streptomyces, which can provide a new source for the research and development of new antibiotics drugs.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： Callicarpa integerrima has very good effect in emoving blood stasis and resolving static blood and treatment of rheumatism evil. However, there are few reports on its chemical composition. In order to explore the chemical composition from the roots and stems of C. integerrima, the 95% ethanol extract of C. integerrima roots and stems were isolated and purified by diverse column chromatography, such as silica gel, sephadex LH-20 gel column, ODS column chromatography, and preparative HPLC. In addition, these compounds were identified on the basis of NMR, ESI-MS as well as other modern spectral techniques. The results were as follows: A total of 15 compounds were isolated from the 95% ethanol extract of the roots and stems of C. integerrima, which were identified as stigmast-4-en-3-one (1), (24R) -5α-stigmastane-3, 6-dione (2), 2'-hydroxy-4'-methoxydihydrochalcone (3), α-amyrin (4), β-sitosterol (5), ursolic acid (6), 4-hydroxy-3-methoxy-benzoic acid (7), 4-pyridinol (8), p-hydroxybenzoic acid (9), forsythoside B (10), nepetifosides D (11), isoacteoside (12), acteoside (13), pedicularioside M (14), β-methoxy forsythoside B (15). All compounds, except for compounds 4-6 and 12-13, were isolated from C. integerrima for the first time. Furthermore, compounds 1, 2, 3, 8, 11 and 14 were isolated from the genus of Callicarpa for the first time.
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