Subjects: Biology >> Botany submitted time 2022-09-03 Cooperative journals: 《广西植物》
Abstract： Carex as a super large genus with high species diversity in the world, 100 species of Carex have been recorded from Guangxi at present. The resources of Carex in Guangxi are still unclear, the in-depth investigations and researches are lack too. Based on specimens identification, literature research and field investigation, we systematically cleared up the early researches of Carex in Guangxi, in order to provide background data for the subsequent research and sustainable utilization. At first, the research brief histories of Carex taxonomy in Guangxi were summarized. The genus Carex in Guangxi with high endemism, we focus on 16 taxa of Carex published based on types collected from Guangxi, verify the accuracy of their type locality, supplement distribution information, and make sure that 8 species of Carex endemic to Guangxi at last. Eight newly recorded species of Carex in Guangxi are reported here, which are C. alopecuroides D. Don, C. chungii Z. P. Wang, C. hirtiutriculata L. K. Dai, C. insignis Boott, C. nubigena D. Don, C. sendaica Franch., C. sociata Boott and C. transversa Boott. The morphological feature diagnosis and voucher specimens for these newly recorded species were provided. We also discussed the species diversity, endemism, geographical distribution, and the potential utilization of Carex in Guangxi.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： TissuecultureandrapidpropagationtechniqueofCalotropisgiganteaL.wasstudied byusingstemsegmentsasexplantsinthispaper.Theresultsshowedthattheoptimalsterilization 32.3%. The suitable primary induction medium was MS+ sucrose 30g稬-1+ ager 3.5 g稬-1. And after 20 d culture, 3-4 cm high regenerative buds were formed. Pre-multiplication culture test showed that MS+ 6-BA1.5 mg稬-1+ NAA0.05 mg稬-1+AgNO31.0 g稬-1+ sucrose 30 g稬-1+ ager 3.5 g稬-1 was a suitable multiplication medium, proliferation coefficient was 4.6/25 d. However, during the subsequent cultivation process, using this method the seedlings were easy to vitrify. With the change of generation, the degree of vitrification has been increasing. So inhibition of vitrification was the key to success.Based on the above multiplication culture test, withAgNO3 as a vitrification inhibitor, the suitable multiplication culture medium of MS+ 6-BA 1.5 mg稬-1+ NAA 0.05 mg稬-1+ AgNO3 1.0 g稬-1+ sucrose 30 g稬-1+ ager 3.5 g稬-1 was verified. With this method, 25 d later, seedling height was 5-8 cm, proliferation coefficient was higher than 5.8/25 d and vitrification rate of multiple shoots was lower than 10%.The optimal medium for rooting was 1/2MS+ NAA1.0 mg稬-1+ sucrose 20 g稬-1 + ager 3.6 g稬-1, 14 d later, the rooting rate was 98%. The rooting seedlings were transplanted in the 70% shading greenhouse.And 30 d later,they were about 20 cm high, with 85% survival rate. This method can be used for the large-scale production ofexcellentclonalseedlingsofCalotropisgiganteaL.
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