Your conditions: 浙江农林大学
  • Combined metabolome and transcriptome analyses reveal the albino mechanism of gold-coloured mutant leaves of Cyclobalanopsis gilva

    Subjects: Biology >> Botany submitted time 2024-06-02 Cooperative journals: 《广西植物》

    Abstract: In order to reveal the albino mechanism of gold-coloured mutant leaves of Cyclobalanopsis gilva, a naturally-occurring leaf-color mutant was used as experimental materials, and the metabolome and transcriptome of mutant leaves and normal green leaves were analyzed by UHPLC-Q Exactive HF-X and high-throughput RNA sequencing, respectively. The results were as follows: ( 1 ) 257 and 357 significantly changed metabolites ( SCMs ) were respectively identified under the positive ion mode and the negative ion mode. Compared with green leaves, the content of some flavonoids such as quercetin, leucoyanidin, myricetin and their derivatives (pyranodelphinin A, isorhamnetin 3-glucuronide, etc. ) increased significantly in mutant leaves, but the content of chlorophylls and carotenoids decreased significantly. ( 2 ) A total of 4 146 differentially expressed genes (DEGs) were detected, of which 1 711 were up-regulated and 2 435 were down-regulated. ( 3 ) KEGG enrichment analysis showed that SCMs and DEGs were mainly enriched in photosynthesis, porphyrin and chlorophyll metabolism and flavonoid biosynthesis. The research results indicate that inhibition of chlorophyll synthesis, chloroplast developmental abnormalities and promotion of flavonoid synthesis were the main factors driving the golden leaf coloration in the mutant C. gilva. In addition, the genes of the MYB and bHLH families were significantly up-regulated in mutant leaves, confirming these two types of transcription factors were involved in regulating flavonoid biosynthesis. This study provided new molecular insights into the phenomenon of leaf etiolation, and also provided reference for exploring leaf color-related functional genes and breeding of landscape plant.

  • Origin, Formation and Development of Discipline of Chemical Processing Engineering of Forest Products

    Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Forestry submitted time 2020-01-13

    Abstract: " This paper introduced the origin, formation and development of the discipline of chemical processing engineering of forest products by analyzing the effect of the fundamental disciplines of chemistry, chemical engineering and biology on the scientific theory and technology development in the area of chemical utilization of forestry plant resources in terms of the basic concepts of discipline science in the past more than 100 years. On these basis, we proposed the idea of the modern discipline of chemical processing engineering of forest products, and elucidated that this modern discipline is concerned with a scientific system of theories and technologies on utilization of forest plant resources by two main approaches of chemistry and bio-chemistry aiming to synthesize and prepare biomass-based energy source, chemicals and functional materials. Finally, we discussed the scientific and technological challenges that the modern discipline of chemical processing engineering of forest products is predominantly faced with in the future.