Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： The circadian clock is a conservative endogenous regulatory mechanism that stimulates and maintains the rhythmic expression of plant physiological characteristics. The PRR proteins are critical components of the circadian clock central oscillator and regulate a variety of life processes such as seed germination and flowering. Anthocyanins are plant secondary metabolites, which play an important role in plant growth, reproduction and stress responses. In this study, we took Arabidopsis thaliana as the research object and explored the function and mechanism of circadian clock PRR proteins in the control of anthocyanin biosynthesis. The results were as follows: (1) The accumulation of anthocyanins and the expression of some anthocyanin synthesis-related genes were significantly reduced in PRR genes single mutant and multiple mutant seedlings. (2) In the seedlings with overexpression of PRR5, the accumulation of anthocyanin and the expression of some anthocyanin synthesis-related genes were significantly increased. (3) The results of the protein-protein interaction experiment showed that PRR5 protein can interact with MYB75, MYB90, MYB113 and TT8 to form protein complexes. (4) Results of genetic analysis showed that PRR5 promoted anthocyanin synthesis in A. thaliana seedlings was the MYB family anthocyanin regulatory proteins dependent. In conclusion, the circadian clock PRR protein may promote the synthesis and accumulation of anthocyanins in A. thaliana seedlings through the interaction of PRR5 protein with MYB75, TT8.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-10-17 Cooperative journals: 《广西植物》
Abstract：脱落酸（Abscisic acid，ABA）激素是一类重要的生长调节物质，参与调控植物的多种生理过程。花青素（Anthocyanins）是植物次生代谢产生的类黄酮化合物，对植物的生长发育和逆境胁迫响应有着重要的作用。最近几年，关于花青素生物合成的转录因子及合成酶基因已有较多研究，然而控制其合成的信号物质及作用机制仍有待深入探究。本文以拟南芥（Arabidopsis thaliana）为对象探讨了ABA信号对花青素生物合成的调控功能和作用机制。实验结果表明外源施加ABA显著提高野生型幼苗茎尖中花青素的积累。相一致的，ABA能诱导某些与花青素合成相关的转录因子及合成酶基因的表达。此外，遗传学分析发现ABA诱导花青素合成部分依赖于MBW复合体中的核心转录因子，如TTG1、TT8及MYB75等。初步机制研究揭示ABA信号途径中的bZIP类转录因子ABI5能与TTG1、TT8及MYB75等相互作用形成蛋白复合物。综上所述，该研究认为ABA信号诱导拟南芥幼苗中花青素的积累，并可能通过ABI5与MBW复合体协同作用调控花青素的合成。
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