分类： 生物学 >> 神经生物学 提交时间： 2017-07-24
摘要： Although depression-induced altered pain perception has been described in several laboratory and clinical studies, its neurobiological mechanism in the central nervous system, particularly in the spinal dorsal horn remains unclear. In this study, we therefore aimed to clarify whether nociceptive sensitivity of neuropathic pain is altered in the olfactory bulbectomy (OB) model of depression and whether glucocorticoid receptor (GR), which is involved in the etio-pathologic mechanisms of both major depression and neuropathic pain, contributes to these processes in the spinal dorsal horn of male Sprague-Dawley rats. The results showed that mechanical allodynia and thermal hyperalgesia induced by spinal nerve ligation (SNL) were attenuated in OB-SNL rats with decreased spinal GR expression and nuclear translocation, while NOB (non-olfactory bulbectomy)-SNL rats showed an increased spinal GR nuclear translocation. Decreased GR nuclear translocation with normal mechanical nociception and hypoalgesia of thermal nociception were observed in OB-Sham rats, too. Intrathecal injection of GR agonist dexamethasone (4 µg / rat / day for 1 week) eliminated the attenuating effect of depression on the nociceptive hypersensitivity in OB-SNL rats and aggravated neuropathic pain in NOB-SNL rats, associating with the up-regulation of BDNF, TrkB and NR2B expression in the spinal dorsal horn. The present study shows that depression attenuates the mechanical allodynia and thermal hyperalgesia of neuropathic pain and suggests that altered spinal GR-BDNF-TrkB signaling may be one of the reasons for depression-induced hypoalgesia.
分类： 生物学 >> 神经生物学 提交时间： 2017-03-31
摘要： Although depression-induced altered pain perception has been described in several laboratory and clinical studies, its neurobiological mechanism in the central nervous system, particularly in the spinal dorsal horn remains unclear. In this study, we therefore aimed to clarify whether nociceptive sensitivity of neuropathic pain is altered in the olfactory bulbectomy (OB) model of depression and whether glucocorticoid receptor (GR), which is involved in the etio-pathologic mechanisms of both major depression and neuropathic pain, contributes to these processes in the spinal dorsal horn of male Sprague-Dawley rats. The results showed that mechanical allodynia and thermal hyperalgesia induced by spinal nerve ligation (SNL) were attenuated in OB-SNL rats with decreased spinal GR expression and nuclear translocation, while NOB (non-olfactory bulbectomy)-SNL rats showed an increased spinal GR nuclear translocation. Decreased GR nuclear translocation with normal mechanical nociception and hypoalgesia of thermal nociception were observed in OB-Sham rats, too. Intrathecal injection of GR agonist dexamethasone (4 µg / rat / day for 1 week) eliminated the attenuating effect of depression on the nociceptive hypersensitivity in OB-SNL rats and aggravated neuropathic pain in NOB-SNL rats, associating with the up-regulation of BDNF, TrkB and NR2B expression in the spinal dorsal horn. The present study shows that depression attenuates the mechanical allodynia and thermal hyperalgesia of neuropathic pain and suggests that altered spinal GR-BDNF-TrkB signaling may be one of the reasons for depression-induced hypoalgesia.
分类： 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间： 2016-05-11
Liu Ying
Mo Wei-Chuan
Fu Jing-Peng
Liu Ying
He Rong-Qiao
Fu Jing-Peng
Liu Ying
He Rong-Qiao
Ding Hai-Min
Hua Qian
摘要： It has been established that exposure in the hypomagnetic field (HMF), which is one of the environmental factor of outer space, has adverse effects on animal and human behavior and brain function. Thus, it is necessary to develop appropriate counteract strategy to avoid the HMF-induced risks to the health of the astronauts during long-term and long-distance manned space mission. However, the physical and mental effects of the HMF in details still await systematic evaluation and the underlying mechanism remains elusive, so far. In this study, we constructed an HMF animal rearing system (<500 nT) and examined the effects of one-month HMF exposure on the circadian behavior, pain response and emotions in adult male C57BL/6 mice (4 similar to 6 weeks old, (20 +/- 2) g). The control animals were reared in the geomagnetic field (GMF). The HMF-exposed animals exhibited a prolonged alteration of the circadian drinking rhythm and a decrease in general activity, accompanied with an increase in thermal hyperalgesia. But the HMF did not induce obvious depression-like and anxiety-related behaviors. The serum noradrenalin concentration in HMF-exposed mice significantly decreased. These findings indicate that the HMF disturbs the behavior rhythm and the function of endocrine system, which probably leads to the subsequently weakened activities of the animal.
分类： 生物学 >> 生物物理学 提交时间： 2016-05-12
Rao, Jia-Sheng
Zhao, Can
Liu, Zuxiang
Yang, Zhao-Yang
Li, Xiao-Guang
Ma, Manxiu
Liu, Zuxiang
Yang, Zhao-Yang
Li, Xiao-Guang
摘要： Purpose: To investigate the longitudinal brain regional homogeneity (ReHo) changes in nonhuman primate after spinal cord injury (SCI) by resting-state functional magnetic resonance imaging (fMRI). Methods: Three adult female rhesus monkeys underwent unilateral thoracic cord injury. A resting-state fMRI examination was performed in the healthy stage and 4, 8, and 12 weeks after the injury. The ReHo value of each voxel in the monkey brain was calculated and compared between pre- and post-SCI monkeys with paired t test. The regions of interest (ROIs) in the significantly changed ReHo regions were set. The correlations between the ReHo change and the time after injury were also determined. Results: Compared with those in healthy period, the ReHo values of the left premotor cortex and the anterior cingulate cortex (ACC) in post-SCI rhesus monkeys significantly increased in 4-week follow-up examinations. The ReHo values of posterior cingulate cortex, left precuneus, left temporal parietooccipital area, and bilateral superior parietal lobules decreased at 8-week follow-up examinations. In 12-week follow-up examinations, the ReHo values of the left postcentral gyrus, right caudate nucleus, and superior temporal gyrus increased. Correlation analysis showed positive correlations between left ACC and the postoperative time. Conclusion: SCI can change the regional synchronism of brain activity in sensorimotor system and the default mode network. These findings may help us to understand the potential pathophysiological changes in the central nervous system after SCI. (C) 2015 Elsevier Inc. All rights reserved.
分类： 医学、药学 >> 临床医学 提交时间： 2022-07-09 合作期刊: 《中国全科医学》
摘要： 背景 难治性癌痛患者常伴有口服困难，增加了治疗的难度，患者静脉自控镇痛（patient-controlled intravenous analgesia, PCIA）可实现持续有效、安全镇痛。目的 探讨氢吗啡酮、舒芬太尼及吗啡在口服困难合并难治性癌痛PCIA中的疗效及安全性。方法 选取口服困难合并难治性癌痛患者102例，单盲、随机数字表法分为A组（氢吗啡酮）、B组（舒芬太尼）、C组（吗啡），各34例，均使用PCIA。对比3组患者治疗前后的疼痛评分、爆发痛发作次数、阿片类药物用量、睡眠质量、生活质量及副作用。结果 在NRS评分及爆发痛发作次数上，治疗后24h、72h时A组显著低于B组和C组（P＜0.05），B组和C组无显著性差异（P＞0.05），治疗后1周、2周、1月时A组显著低于C组（P＜0.05），A组与B组无显著性差异（P＞0.05），治疗后2周、1月时B组显著低于C组（P＜0.05）；治疗后1周，B组和C组的爆发痛发作次数无显著性差异（P＞0.05），B组的NRS评分显著低于C组（P＜0.05）。治疗后1个月，A组和B组的每日等效口服吗啡剂量（morphine equivalent daily dose, MEDD）均显著低于C组，A组显著低于B组（均P＜0.05），A组和B组的PSQI评分无显著性差异（P＞0.05），且均显著低于C组（P＜0.05）。治疗后1个月，A组仅在情绪功能评分上显著高于B组，A组和B组各项生活质量评分均显著高于C组（均P＜0.05）。治疗后1个月，3组副作用无显著性差异（P＞0.05）。结论 对口服困难合并难治性癌痛患者使用PCIA，相比吗啡，氢吗啡酮、舒芬太尼均能更有效降低疼痛评分、减少爆发痛发作次数、改善睡眠、提高生活质量，但氢吗啡酮比舒芬太尼起效更快，在改善情绪方面效果更佳，阿片类药物用量更低，且不增加副作用。
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