分类： 生物学 >> 生物物理学 >> 细胞生物学 提交时间： 2016-05-12
摘要： PGE2 elevates IL-23 production in mouse dendritic cells while inhibits IL-23 production in isolated human monocytes. Whether this differential effect of PGE2 on IL-23 production is cell-type-or species-specific has not been investigated in detail. The present study was designed to investigate the effect of PGE2 on IL-23 production in human DCs and the possible underlying mechanisms. Human monocytes derived dendritic cells (Mo-DCs) were pretreated with or without PGE2. Then the cells were incubated with zymosan. Our results demonstrated that PGE2 promoted zymosan-induced IL-23 production in a concentration dependent manner. In addition, it was found that PGE2 is also able to elevate MyD88-mediated IL-23 p19 promoter activity. More importantly, ELISA data demonstrated that db-cAMP, a cAMP analog, and forskolin, an adenylate cyclase activator, can mimic the effect of PGE2 on zymosan-induced IL-23 production, and rp-cAMP, a protein kinase A (PKA) inhibitor, can block the effect of PGE2. Moreover, PGE2 can increase zymosan-induced expression of the mRNA levels of both p19 and p40 subunits, which was mimicked by db-cAMP and forskolin. Our data suggest that PGE2 elevates the production of IL-23 in humanMo-DCs via a cAMP dependent pathway.
分类： 生物学 >> 生物物理学 提交时间： 2016-05-15
摘要： Background Cilostazol, an anti-platelet drug for treating coronary heart disease, has been reported to modulate immune cell functions. Plasmacytoid dendritic cells (pDCs) have been found to participate in the progression of atherosclerosis mainly through interferon a (IFN-alpha) production. Whether cilostazol influences pDCs activation is still not clear. In this study, we aimed to investigate the effects of cilostazol on cell activation and antigen presentation of pDCs in vitro in this study. Methods Peripheral blood mononuclear cells isolated by Ficoll centrifugation and pDCs sorted by flow cytometry were used in this study. After pretreated with cilostazol for 2 h, cells were stimulated with CpG-A, R848 or virus for 6 h or 20 h, or stimulated with CpG-B for 48 h and then co-cultured with naive T cell for five days. Cytokines in supernatant and intracellular cytokines were analyzed by ELISA or flow cytometry respectively. Results Our data indicated that cilostazol could inhibit IFN-alpha and tumor necrosis factor a (TNF-alpha) production from pDCs in a dose-dependent manner. In addition, the ability of priming naive T cells of pDCs was also impaired by cilostazol. The inhibitory effect was not due to cell killing since the viability of pDCs did not change upon cilostazol treatment. Conclusion Cilostazol inhibits pDCs cell activation and antigen presentation in vitro, which may explain how cilostazol protects against atherosclerosis.
分类： 物理学 >> 地球物理学、天文学和天体物理学 提交时间： 2024-02-01 合作期刊: 《Research in Astronomy and Astrophysics》
摘要： Very low frequency (VLF) signals are propagated between the ground-ionosphere. Multimode interference will cause the phase to show oscillatory changes with distance while propagating at night, leading to abnormalities in the received VLF signal. This study uses the VLF signal received in Qingdao City, Shandong Province, from the Russian Alpha navigation system to explore the multimode interference problem of VLF signal propagation. The characteristics of the effect of multimode interference phenomena on the phase are analyzed according to the variation of the phase of the VLF signal. However, the phase of VLF signals will also be affected by the X-ray and energetic particles that are released during the eruption of solar flares, therefore the two phenomena are studied in this work. It is concluded that the X-ray will not affect the phase of VLF signals at night, but the energetic particles will affect the phase change, and the influence of energetic particles should be excluded in the study of multimode interference phenomena. Using VLF signals for navigation positioning in degraded or unavailable GPS conditions is of great practical significance for VLF navigation systems as it can avoid the influence of multimode interference and improve positioning accuracy.
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