分类: 生物学 >> 生物物理学 >> 细胞生物学 提交时间: 2016-05-12
摘要: Nuclease-based gene editing technologies have opened up opportunities for correcting human genetic diseases. For the first time, scientists achieved targeted gene editing of mitochondrial DNA in mouse oocytes fused with patient cells. This fascinating progression may encourage the development of novel therapy for human maternally inherent mitochondrial diseases.
分类: 物理学 >> 核物理学 提交时间: 2016-09-01
摘要: It is proposed to upgrade the endcap time-of-flight (ETOF) of the Beijing Spectrometer III (BESIII) with multi-gap resistive plate chamber (MRPC), aiming at overall time resolution about 80 ps. After the entire electronics system is ready, some experiments, such as heat radiating, irradiation hardness and large-current beam tests,are carried out to certify the electronics' reliability and stability. The on-detector test of the electronics is also performed with the beam at BEPCII E3 line, the test results indicate that the electronics system fulfills its design requirements.
分类: 核科学技术 >> 粒子加速器 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要: The macromolecular crystallography beamline BL17U1 at the Shanghai Synchrotron Radiation Facility (SSRF) is the first dedicated macromolecular crystallography (MX) beamline at a third-generation synchrotron in China. It utilizes an in-vacuum undulator as a source and is energy-tunable from 5 to 18 keV. The beamline was commissioned and opened for users in April 2009. The experimental station was upgraded in 2011 with an advanced detector, a high precision goniometer and an automatic sample exchanger for high efficient and high-throughput data collection of protein crystals. The current set-up allows for remote operation of sample mounting, centering and data collection of pre-frozen crystals. In recent two years, the number of PDB depositions from this beamline exceeds 330 each year. In this paper, we describe the complete BL17U1 beamline with upgraded end station and how it is managed for user community.
分类: 生物学 >> 生物物理学 >> 细胞生物学 提交时间: 2016-05-05
摘要: Xeroderma pigmentosum (XP) is a group of genetic disorders caused by mutations of XP-associated genes, resulting in impairment of DNA repair. XP patients frequently exhibit neurological degeneration, but the underlying mechanism is unknown, in part due to lack of proper disease models. Here, we generated patient-specific induced pluripotent stem cells (iPSCs) harboring mutations in five different XP genes including XPA, XPB, XPC, XPG, and XPV. These iPSCs were further differentiated to neural cells, and their susceptibility to DNA damage stress was investigated. Mutation of XPA in either neural stem cells (NSCs) or neurons resulted in severe DNA damage repair defects, and these neural cells with mutant XPA were hyper-sensitive to DNA damage-induced apoptosis. Thus, XP-mutant neural cells represent valuable tools to clarify the molecular mechanisms of neurological abnormalities in the XP patients.
分类: 物理学 >> 核物理学 提交时间: 2016-09-14
摘要: In order to improve the particle identification capability of the Beijing Spectrometer III (BESIII),t is proposed to upgrade the current endcap time-of-flight (ETOF) detector with multi-gap resistive plate chamber (MRPC) technology. Aiming at extending ETOF overall time resolution better than 100ps, the whole system including MRPC detectors, new-designed Front End Electronics (FEE), CLOCK module, fast control boards and time to digital modules (TDIG), was built up and operated online 3 months under the cosmic ray. The main purposes of cosmic ray test are checking the detectors' construction quality, testing the joint operation of all instruments and guaranteeing the performance of the system. The results imply MRPC time resolution better than 100ps, efficiency is about 98%燼nd the noise rate of strip is lower than 1Hz/(scm2) at normal threshold range, the details are discussed and analyzed specifically in this paper. The test indicates that the whole ETOF system would work well and satisfy the requirements of upgrade.