分类: 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间: 2016-05-12
摘要: With the aim of broadening the versatility of lentiviral vectors as a tool in nucleic acid research, we expanded the genetic code in the propagation of lentiviral vectors for site-specific incorporation of chemical moieties with unique properties. Through systematic exploration of the structure-function relationship of lentiviral VSVg envelope by site-specific mutagenesis and incorporation of residues displaying azide- and diazirine-moieties, the modifiable sites on the vector surface were identified, with most at the PH domain that neither affects the expression of envelope protein nor propagation or infectivity of the progeny virus. Furthermore, via the incorporation of such chemical moieties, a variety of fluorescence probes, ligands, PEG and other functional molecules are conjugated, orthogonally and stoichiometrically, to the lentiviral vector. Using this methodology, a facile platform is established that is useful for tracking virus movement, targeting gene delivery and detecting virus-host interactions. This study may provide a new direction for rational design of lentiviral vectors, with significant impact on both basic research and therapeutic applications.
分类: 核科学技术 >> 粒子加速器 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要: The macromolecular crystallography beamline BL17U1 at the Shanghai Synchrotron Radiation Facility (SSRF) is the first dedicated macromolecular crystallography (MX) beamline at a third-generation synchrotron in China. It utilizes an in-vacuum undulator as a source and is energy-tunable from 5 to 18 keV. The beamline was commissioned and opened for users in April 2009. The experimental station was upgraded in 2011 with an advanced detector, a high precision goniometer and an automatic sample exchanger for high efficient and high-throughput data collection of protein crystals. The current set-up allows for remote operation of sample mounting, centering and data collection of pre-frozen crystals. In recent two years, the number of PDB depositions from this beamline exceeds 330 each year. In this paper, we describe the complete BL17U1 beamline with upgraded end station and how it is managed for user community.