分类: 核科学技术 >> 核材料与工艺技术 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要: In this paper, the safety and efficacy of 131I-labeled mouse/human chimeric monoclonal antibody (131I-chTNT)-mediated radioimmunotherapy are evaluated because the patients have non-uptaking 131I pulmonary metastases from differentiated thyroid carcinoma (DTC). The 16 patients were injected intravenously by 29.63.7 MBqkg1 using 131I-chTNT. The chest computer tomography was performed before treatment, as well as 28 and 70 days after treatment. Responses and safety were assessed during the treatment. The results show that the 131I-chTNT infusion was well tolerated with the 12.5% complete response, 18.8% partial response, 25.0% progressive disease, and the 43.8% stable disease, indicating that most treatment-related adverse effects are mild transient and reversible. The 131I-chTNT is promising for patients with non-uptaking the 131I pulmonary metastases from DTC.
分类: 地球科学 >> 地理学 提交时间: 2021-06-04 合作期刊: 《干旱区科学》
摘要: Drought is an inevitable condition with negative impacts in the agricultural and climatic sectors, especially in developing countries. This study attempts to examine the spatial and temporal characteristics of drought and its trends in the Koshi River Basin (KRB) in Nepal, using the standardized precipitation evapotranspiration index (SPEI) over the period from 1987 to 2017. The Mann-Kendall test was used to explore the trends of the SPEI values. The study illustrated the increasing annual and seasonal drought trends in the KRB over the study period. Spatially, the hill region of the KRB showed substantial increasing drought trends at the annual and seasonal scales, especially in summer and winter. The mountain region also showed a significant increasing drought trend in winter. The drought characteristic analysis indicated that the maximum duration, intensity, and severity of drought events were observed in the KRB after 2000. The Terai region presented the highest drought frequency and intensity, while the hill region presented the longest maximum drought duration. Moreover, the spatial extent of drought showed a significant increasing trend in the hill region at the monthly (drought station proportion of 7.6%/10a in August), seasonal (drought station proportion of 7.2%/10a in summer), and annual (drought station proportion of 6.7%/10a) scales. The findings of this study can assist local governments, planners, and project implementers in understanding drought and developing appropriate mitigation strategies to cope with its impacts.
分类: 生物学 >> 生物物理学 >> 细胞生物学 提交时间: 2016-05-11
摘要: Many receptor-mediated endocytic processes are mediated by constitutive budding of clathrin-coated pits (CCPs) at spatially randomized sites before slowly pinching off from the plasma membrane (60100 s). In contrast, clathrin-mediated endocytosis (CME) coupled with regulated exocytosis in excitable cells occurs at peri-exocytic sites shortly after vesicle fusion (similar to 10 s). The molecular mechanism underlying this spatiotemporal coupling remains elusive. We show that coupled endocytosis makes use of preformed CCPs, which hop to nascent fusion sites nearby following vesicle exocytosis. A dynamic cortical microtubular network, anchored at the cell surface by the cytoplasmic linker-associated protein on microtubules and the LL5 beta/ELKS complex on the plasma membrane, provides the track for CCP hopping. Local diacylglycerol gradients generated upon exocytosis guide the direction of hopping. Overall, the CCP-cytoskeleton-lipid interaction demonstrated here mediates exocytosis-coupled fast recycling of both plasma membrane and vesicular proteins, and it is required for the sustained exocytosis during repetitive stimulations.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-12
摘要: Tetrapyrroles, including haem and chlorophyll, play vital roles for various biological processes, such as respiration and photosynthesis, and their biosynthesis is critical for virtually all organisms. In photosynthetic organisms, magnesium chelatase (MgCh) catalyses insertion of magnesium into the centre of protoporphyrin IX, the branch-point precursor for both haem and chlorophyll, leading tetrapyrrole biosynthesis into the magnesium branch1,2. This reaction needs a cooperated action of the three subunits of MgCh: the catalytic subunit ChlH and two AAA(+) subunits, ChlI and ChlD ( refs 3-5). To date, the mechanism of MgCh awaits further elucidation due to a lack of high-resolution structures, especially for the similar to 150 kDa catalytic subunit. Here we report the crystal structure of ChlH from the photosynthetic cyanobacterium Synechocystis PCC 6803, solved at 2.5 angstrom resolution. The active site is buried deeply inside the protein interior, and the surrounding residues are conserved throughout evolution. This structure helps to explain the loss of function reported for the cch and gun5 mutations of the ChlH subunit, and to provide the molecular basis of substrate channelling during the magnesium-chelating process. The structure advances our understanding of the holoenzyme of MgCh, a metal chelating enzyme other than ferrochelatase.