分类: 生物学 >> 生物物理学 提交时间: 2016-05-12
摘要: We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more photons under our cryogenic imaging condition, resulting in higher localization precision which is comparable to ambient super-resolution imaging. Vitrified specimens were prepared by high pressure freezing and cryo-sectioning to maintain a near-native state with better fluorescence preservation. A 2-3-fold improvement of resolution over the recent reports was achieved due to the photon budget performance of screening out Dronpa and optimized imaging conditions, even with thin sections which is at a disadvantage when calculate the structure resolution from label density. We extended csCLEM to mammalian cells by introducing cryo-sectioning and observed good correlation of a mitochondrial protein with the mitochondrial outer membrane at nanometer resolution in three dimensions.
分类: 天文学 >> 天文学 提交时间: 2023-02-19
摘要: The radius valley, a dip in the radius distribution of exoplanets at ~1.9 Earth radii separates compact rocky Super-Earths and Sub-Neptunes with lower density. Various hypotheses have been put forward to explain the radius valley. Characterizing the radius valley morphology and its correlation to stellar properties will provide crucial observation constraints on its origin mechanism and deepen the understanding of planet formation and evolution. In this paper, the third part of the Planets Across the Space and Time (PAST) series, using the LAMOST-Gaia-Kepler catalog, we perform a systematical investigation into how the radius valley morphology varies in the Galactic context, i.e., thin/thick galactic disks, stellar age and metallicity abundance ([Fe/H] and [alpha/Fe]). We find that (1) The valley becomes more prominent with the increase of both age and [Fe/H]. (2) The number ratio of super-Earths to sub-Neptunes monotonically increases with age but decreases with [Fe/H] and [alpha/Fe]. (3) The average radius of planets above the valley (2.1-6 Earth radii) decreases with age but increases with [Fe/H]. (4) In contrast, the average radius of planets below the valley (R < 1.7 Earth radii) is broadly independent on age and metallicity. Our results demonstrate that the valley morphology as well as the whole planetary radius distribution evolves on a long timescale of giga-years, and metallicities (not only Fe but also other metal elements, e.g., Mg, Si, Ca, Ti) play important roles in planet formation and in the long term planetary evolution.
分类: 天文学 >> 天文学 提交时间: 2023-02-19
摘要: The Kepler telescope has discovered over 4,000 planets (candidates) by searching ? 200,000 stars over a wide range of distance (order of kpc) in our Galaxy. Characterizing the kinematic properties (e.g., Galactic component membership and kinematic age) of these Kepler targets (including the planet (candidate) hosts) is the first step towards studying Kepler planets in the Galactic context, which will reveal fresh insights into planet formation and evolution. In this paper, the second part of the Planets Across the Space and Time (PAST) series, by combining the data from LAMOST and Gaia and then applying the revised kinematic methods from PAST I, we present a catalog of kinematic properties(i.e., Galactic positions, velocities, and the relative membership probabilities among the thin disk, thick disk, Hercules stream, and the halo) as well as other basic stellar parameters for 35,835 Kepler stars. Further analyses of the LAMOST-Gaia-Kepler catalog demonstrate that our derived kinematic age reveals the expected stellar activity-age trend. Furthermore, we find that the fraction of thin(thick) disk stars increases (decreases) with the transiting planet multiplicity (Np = 0, 1, 2 and 3+) and the kinematic age decreases with Np, which could be a consequence of the dynamical evolution of planetary architecture with age. The LAMOST-Gaia-Kepler catalog will be useful for future studies on the correlations between the exoplanet distributions and the stellar Galactic environments as well as ages.
分类: 天文学 >> 天文学 提交时间: 2023-02-19
摘要: We propose to develop a wide-field and ultra-high-precision photometric survey mission, temporarily named "Earth 2.0 (ET)". This mission is designed to measure, for the first time, the occurrence rate and the orbital distributions of Earth-sized planets. ET consists of seven 30cm telescopes, to be launched to the Earth-Sun's L2 point. Six of these are transit telescopes with a field of view of 500 square degrees. Staring in the direction that encompasses the original Kepler field for four continuous years, this monitoring will return tens of thousands of transiting planets, including the elusive Earth twins orbiting solar-type stars. The seventh telescope is a 30cm microlensing telescope that will monitor an area of 4 square degrees toward the galactic bulge. This, combined with simultaneous ground-based KMTNet observations, will measure masses for hundreds of long-period and free-floating planets. Together, the transit and the microlensing telescopes will revolutionize our understandings of terrestrial planets across a large swath of orbital distances and free space. In addition, the survey data will also facilitate studies in the fields of asteroseismology, Galactic archeology, time-domain sciences, and black holes in binaries.