• Beautiful Stories with the Library for the Past 40 Years: An Oral Interview with Readers of Guangzhou Library

    Subjects: Other Disciplines >> Synthetic discipline submitted time 2024-06-13 Cooperative journals: 《高校图书馆工作》

    Abstract: In celebration of the 40th anniversary, Guangzhou Library conducted oral interviews with people from all walks of life who participated in the construction and have been supporting the development of the library. Among those interviewees, there are four readers with different career backgrounds and interests: Huang Lihai (a poet), Chen Keng (an artist), Zhang Wendong (an activist of public welfare for the visual-impaired) and He Qiwei (an office worker). In the company of Guangzhou Library, they have found their own life directions and growing paths and witnessed great changes of the library over the past 40 years. This interview article presents four readers’ impressive stories and expectations on Guangzhou Library, revealing a public library’s development track and spiritual nourishment for the city and citizens from readers’ perspective.

  • 饲粮能量和蛋白质水平对滩羊小肠中小肽和氨基酸转运载体mRNA表达量的影响

    Subjects: Biology >> Zoology submitted time 2017-10-23 Cooperative journals: 《动物营养学报》

    Abstract:本试验旨在研究饲粮能量和蛋白质水平对滩羊小肠中小肽和氨基酸转运载体mRNA表达量的影响。选取112只健康、体重相近的滩羊,随机分成4组,每组4个重复,每个重复7只羊。标准水平的饲粮能量和蛋白质水平参考《肉羊饲养标准》(NY/T 816—2004),各组试验滩羊分别饲喂不同能量和蛋白质水平饲粮:0.84×标准水平(Ⅰ组)、0.96×标准水平(Ⅱ组)、1.08×标准水平(Ⅲ组)和1.20×标准水平(Ⅳ组)。试验根据羊体重分2个阶段:29~35 kg和36~40 kg。于每个阶段末,每个重复屠宰1只试验羊,取其小肠组织样,运用实时荧光定量PCR技术,研究小肽转运载体1(PepT1)、y+型氨基酸转运载体1(CAT1)、兴奋性氨基酸转运载体3(EAAT3)mRNA表达量的变化。结果表明:1)在29~35 kg阶段末,小肠中PepT1 mRNA的表达量随着饲粮能量和蛋白质水平的提高呈先下降再上升的趋势,Ⅱ组显著低于其他3组(P<0.05);Ⅳ组小肠中CAT1 mRNA的表达量显著高于其他3组(P<0.05);Ⅲ组小肠中EAAT3 mRNA的表达量显著高于其他3组(P<0.05)。2)在36~40 kg阶段末,Ⅱ组小肠中PepT1 mRNA的表达量显著高于其他3组(P<0.05);Ⅱ组小肠中CAT1 mRNA的表达量显著高于Ⅲ组(P<0.05);小肠中EAAT3 mRNA的表达量随着饲粮能量和蛋白质水平的提高呈上升趋势,Ⅲ组和Ⅳ组小肠中EAAT3 mRNA的表达量显著高于Ⅰ组和Ⅱ组(P<0.05)。由此可见,饲粮能量和蛋白质水平会影响滩羊小肠中PepT1、CAT1、EAAT3 mRNA的表达量,使机体对小肽和氨基酸的吸收利用率随之改变,以适应滩羊的生长发育。