Subjects: Biology >> Botany >> Applied botany submitted time 2020-04-03 Cooperative journals: 《广西植物》
Abstract：龙胆苦苷（gentiopicroside）是中药龙胆中的主要药效成分，属于萜类化合物的衍生物。1- 羟基-2- 甲基-2- （ E ） - 丁烯基-4- 二磷酸还原酶（ 1-hydroxy-2-methyl-2-butenyl-4-diphosphate reductase），HDR 是萜类物质合成途径中的关键酶。为探讨不同光照条件下滇龙胆HDR（GrHDR）基因的表达与龙胆苦苷含量之间的关系，以滇龙胆叶片cDNA 为模板，采用PCR 和TA 克隆技术获得GrHDR 基因序列，对该序列进行生物信息学分析和表达分析，并采用高效液相色谱法测定龙胆苦苷含量，对该基因表达与龙胆苦苷含量进行比较。结果表明：GrHDR 基因（GenBank 登录号： KJ917165.1）全长1 398 bp，编码465 个氨基酸，推定GrHDR 蛋白是亲水且稳定的，相对分子质量是52 281.25 Da，理论等电点是5.32；该蛋白属于LYTB 蛋白家族，可能定位于叶绿体上，无信号肽，二级结构主要由α-螺旋（45.16%），β-转角（6.24%）、无规卷曲（33.98%）、延伸链（14.62%）构成；GrHDR 蛋白序列与同属植物秦艽的HDR 蛋白相似性最高（95.71%）；实时荧光定量PCR 结果显示GrHDR 基因在滇龙胆中的表达量为：根> 叶> 茎，而在10%、30%、100%全光光照条件下各组织的表达量有很大差异；高效液相色谱法结果显示，不同光照条件下龙胆苦苷含量一致，均为：根> 叶> 茎，其中100%全光光照下，药用部位根中龙胆苦苷含量达到7.141%，约是30%、10%全光光照条件的两倍，但该结果与同一光照条件下GrHDR 基因表达规律不完全一致。本研究为阐述HDR 基因功能及其与龙胆苦苷含量的关系提供参考。
Subjects: Geosciences >> Geography submitted time 2024-03-01 Cooperative journals: 《干旱区研究》
Abstract： Due in large part to global climate change, drought, flood, and high temperature events have increasedsignificantly around the world in recent years. The Shiyang River Basin is in Northwest China and fringes onto amonsoon region, and is consequently, highly sensitive to climate change. The rapid development of oasisagriculture has led to high levels of development and the utilization of water resources in fragile ecologicalenvironments. Future climate change will aggravate the uncertainty of water resources in the basin, posing athreat to food security and economic development. Coupled General Circulation Models (GCMs) play animportant role in the prediction of future climate change and formulation strategies to help devise adjustmentsaccordingly. Based on the observed data in the historical period (1985-2014), the simulation capabilities of 11climate models from the 6th international Coupled Model Intercomparison Program (CMIP6) in the ShiyangRiver Basin were evaluated. The equidistant cumulative distribution function method was applied to downscaleclimate data to obtain the future climate change trend for the basin as presented in this paper. The results showthat the CMIP6 multi-model ensemble has good applicability in the Shiyang River Basin, as it accurately depictsthe annual and seasonal distribution characteristics of climate factors, including precipitation, temperature, andpotential evapotranspiration. The model performs well when simulating temperatures, in comparison toprecipitation. While multimodel ensemble mean data perform better when simulating precipitation andtemperature in the Shiyang River Basin, in comparison with other models. Under different future scenarios (2023-2100), precipitation, temperature, and potential evapotranspiration in the basin show a significant upward trendand increase with the radiative forcing increase. The late 21 century shows a greater increase in climate factorsthan the early and middle periods. Compared to the historical period, precipitation in the future could increase by45.02% in the winter and 0.38% in the summer, and the greatest temperature increases can occur in spring andautumn. In the future, the aridity index of the Shiyang River Basin will decrease overall. The climate of the basinwill tend to warm and humidify, with the summer season becoming drier while the other seasons become wetterthan those in the historical period. The Minqin Basin located in the lower reaches of the basin is the area mostsensitive to climate change. The research results have important reference value as they will help to addressfuture climate change and ensure sustainable economic and agricultural development in the Shiyang River Basin.
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