Subjects: Biology >> Biochemistry submitted time 2023-02-24
Abstract： This study aimed to explore the effect of massage on the urine proteome of healthy people. In this study, participants underwent 1-hour whole body massage. Urine samples were collected at 0, 2, and 24h after the massage and urine proteins were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Compared with the control (before massage), 41 differential proteins were identified in the group 2h after the massage, the random mean number of differentially produced proteins was 11 with 73% confidence, and the biological process of protein enrichment was catecholamine biosynthesis, which was related to the promotion of metabolism and the regulation of neural activities. While 29 differential proteins were identified in the group 24h after the massage, the random average number of differential proteins produced was 10, with the confidence of the difference decreased to 65%, and the effective biological process could not be enriched at this time. The results suggested that the difference in urine protein was greater at 2h after the massage and gradually decreased at 24h after the massage. The proteome of urine may reflect changes in the body following minor massage stimuli, providing a potential way to evaluate the effects of massage therapy.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-04-24
Abstract：
[Objective] To explore the effect of traditional Chinese medicine on the urine proteome of healthy rats.
[Methods] In this study, Intragastric administration rat models were established by two traditional Chinese medicines (Compound Danshen Dropping Pills and Huoxiangzhengqi Oral Liquid). Urine samples were collected from rats before and after intragastric administration. Liquid chromatography-mass spectrometry (LC-MS/MS) was used to screen the urine differential proteins of rats before and after intragastric administration and analyze the biological pathway of differential proteins.
[Results] Urine proteome can reflect the changes before and after 14 days of intragastric administration of Compound Danshen Dropping Pills, and the biological pathways enriched by differential proteins are related to their mechanism of action in the treatment of cardiovascular diseases, such as glycolysis, lipid metabolism.
[Conclusions] Urine proteomics can directly and systematically reflect the overall impact of traditional Chinese medicine on the body, and provides a new method to study the efficacy of traditional Chinese medicine.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-03-09
Abstract：
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Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-03-03
Abstract：
Abstract：
[Objective] To investigate the effect of hypoxia on urine proteome in rats,and to find the changes of urine protein related to hypoxia stress.
[Methods] In this study,liquid chromatography-mass spectrometry (LC-MS/MS) was used to screen the urine differential proteins of rats with 12 and 24h hypoxia and analyze the biological pathway to observe the changes of urine protein in rats with acute hypoxia stress.
[Results] We found that the urine proteome could clearly distinguish the normoxic and hypoxic samples, and the differential proteins were also enriched in biological pathways related to hypoxic stress,such as antioxidant stress,glycolysis,complement and coagulation cascade.
[Conclusions] Urine proteome can reflect the significant changes after acute hypoxic stimulation, which is helpful for the detection of hypoxia.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-05-25
Abstract： Drug addiction is a serious chronic relapsing brain disease, and methamphetamine dependence has a complex course and is difficult to treat, causing a serious public health burden. In this study, we compared and analyzed the urine proteome of methamphetamine-using patients (patients who stopped using methamphetamine within 24 hours), methamphetamine-withdrawing patients (patients who stopped using drugs for more than 3 months and entered rehabilitation treatment), healthy individuals. The urine proteome of methamphetamine-addicted patients (including patients who were using methamphetamine and methamphetamine-withdrawing patients) was significantly different from that of healthy individuals, and some differential proteins and their enriched biological functions showed that they were associated with addiction or neurotoxicity of methamphetamine and may be potential intervention targets for drug addiction. Patients who withdrew methamphetamine for more than 3 months were still difficult to return to normal levels and may be used to reveal the reasons for the high rate of methamphetamine relapse. In this study, a method of studying addictive drugs from the perspective of urine proteomics was established, which proved that urine proteome can reflect the effects of methamphetamine abuse on the body relatively systematically and comprehensively, and has the potential to provide clues for the study and practice of clinical addictive diseases.
Peer Review Status:Awaiting Review
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