Subjects: Biology >> Botany >> Applied botany submitted time 2022-05-21 Cooperative journals: 《广西植物》
Abstract： In order to explore the material basis of the root bark of root bark of Ailanthus altissima antibacterial activity, the silica gel and Sephadex LH-20 were employed to separate and purify methanol extract from the root bark of the A. altissima, and the structure of the compounds were identified by chemical properties and spectral data. Flow cytometry was employed to test the antibacterial activity of the compounds, and kanamycin was used as the control group. The results were as follows: Twenty-two compounds were isolated and elucidated from the root bark of A. altissima respectively as pleuchiol (1), withastramonolide (2), 7-ketositosterol (3), betulin (4), betulinic acid methyl ester (5), 1, 2, 4-trimethoxybenzene (6), dimethyl maleate (7), sonderianol (8), dibutyl phthalate (9), pinoresinol (10), p-hydroxybenzoic acid ethyl ester (11), avenalumic acid methyl ester (12), 5, 3'-dihydroxy-3,7, 4'-trimethoxyflavone (13), spathulenol (14), 2-methyl-5-acetonyl-7-hydroxychromone (15), 7, 4'-dihydroxyflavone (16), annphenone (17), 3-hydroxy-4-methoxy-benzoic acid (18), 5, 3',4'-trihydroxy-7-methoxyflavanone (19), dibutyl phthalate (20), 4-O-methylgallic acid (21), dioctyl terephthalate (22). All compounds were isolated from the root bark of A. altissima for the first time. The antibacterial activity tests showed that compound 2 had inhibitory effects on Pseudomonas aeruginosa and Bacillus subtilis. Compound 3 had inhibitory effects on Bacillus subtilis. Compound 8 had inhibitory effects on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Compound 17 had inhibitory effects on Pseudomonas aeruginosa and Staphylococcus aureus. The inhibitory effect of compound 2 on Bacillus subtilis was not significantly different from kanamycin (P＞0.05). This paper aims to clarify the bacteriostatic substance basis of the root bark of the root bark of A. altissima, and provide a certain theoretical basis for the development and utilization of the root bark of the root bark of A. altissima resources and the research and development of drugs with antibacterial activity.
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