Subjects: Biology >> Botany >> Applied botany submitted time 2023-08-04 Cooperative journals: 《广西植物》
Abstract： Water deficiency is a critical factor that restricts the utilization of Rhododendron delavayi in landscaping. bHLH transcription factors are pivotal regulators of plant growth, development, and stress responses. This study aimed to identify members of the R. delavayi bHLH transcription factor (RdbHLH) family by utilizing the R. delavayi genome file and transcriptome data. Through bioinformatics methods, we analyzed various aspects, including gene structure, conserved motifs, phylogenetic relationships, protein physicochemical properties, cis-acting elements, protein interaction networks, and expression patterns. The results were as follows: (1) A total of 116 RdbHLH genes were identified, exhibiting significant variations in terms of amino acid composition and molecular weight. These genes are primarily weakly acidic hydrophilic proteins that function predominantly within the cell nucleus. (2) The RdbHLH family was categorized into 17 subfamilies, where motif structures were conserved within each subfamily but displayed noticeable differences between different subfamilies. Most RdbHLH proteins contained both Motif 1 and Motif 2. The promoter region of these genes contained a substantial number of cis-acting elements associated with plant growth and development, hormone response, light response, and stress response. (3) R. delavayi responds to drought stress by activating signal transduction pathways and regulating osmotic pressure and flavonoid biosynthesis systems, leading to stress damage mitigation. Drought stress exerted an impact on the expression of 36 RdbHLH genes, with 12 genes displaying robust induction. Notably, RdbHLH49 and RdbHLH95 may play pivotal regulatory roles in enhancing plant resistance to drought stress. These research findings provide a theoretical basis for further investigations into the biological functions of RdbHLH genes and establish target gene resources for the breeding of superior horticultural varieties of R. delavayi.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-07-31 Cooperative journals: 《广西植物》
Abstract： NAC transcription factors play important roles in plant growth, development, and various stress responses. However, the molecular mechanism of the RdNAC72 gene in Rhododendron delavayi involved in the heat stress response was still unclear. To investigate the the roles of the RdNAC72 gene in heat stress response, we first designed primers for cloning the full length coding sequence of the RdNAC72 gene using PCR technology. Subsequently, the gene’s structure, function, and physicochemical properties were analyzed and predicted using bioinformatics method. The spatial and temporal expression characteristics of the RdNAC72 gene under heat stress and ABA were analyzed using real-time fluorescence quantitative PCR(RT-qPCR). The results were as follows: (1) The RdNAC72 gene had a full length of 1 005 bp, encoding 334 amino acids with a relative molecular weight of 37.415 kDa. Subcellular localization analysis showed that the RdNAC72 protein was located in the nucleus. (2) Multiple sequence alignment and phylogenetic analysis indicated that the RdNAC72 was most closely related to the RwNAC72 in R. williamsianum. Additionally, cis-acting element analysis revealed that the gene contains elements associated with hormone response, light response, anaerobic response, low temperature response, and heat stress response. (3) Heat stress could induce the expression of RdNAC72, exhibiting temporal and spatial expression specificity. After three days of heat stress treatment, the relative expression level of the RdNAC72 gene in leaves was significantly upregulated by 31.16-fold, while no significant changes were observed in stems and roots. After six days of heat stress treatment, the relative expression levels of RdNAC72 were significantly upregulated, with the highest observed in leaves (61.56-fold), followed by stems (50.14-fold), and roots (17.42-fold). Additionally, it was found that ABA was found to induce the expression of RdNAC72. (4) RT-qPCR analysis demonstrated a coordinated expression pattern between RdHSP17.2 and RdNAC72 with RdHSP17.2 containing multiple NAC recognition motifs (CATGTG) and core binding sequences (CACG) in its promoter region, suggesting it may be a downstream target gene of RdNAC72. Therefore, the RdNAC72, a transcription factor, localized in the nucleus, responds significantly to high temperatures and ABA, potentially activating the RdHSP17.2 expression to confer heat resistance. These findings not only understanding our comprehension of the biological functions of NAC transcription factors in response to stress, but also potentially guide future genetic and breeding strategies to enhance stress resilience in plants.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-11-04 Cooperative journals: 《广西植物》
Abstract： The TIFY gene family plays a very important role in Camellia sinensis hormone signal transduction and its adversity stress. Bioinformatics methods were employed to identify the TIFY family members in the C. sinensis genome in the study, and the physical and chemical properties, system evolution, gene structure, chromosomal location, the cis-acting elements of promoter region and tissue expression pattern were also analyzed, and the results of quantitative experiments verified the hormone response and stress response characteristics of some members of the TIFY family. The results were as follows: (1)There were 19 TIFY gene members (CSTIFY1-CSTIFY19) in C. sinensis, which belonged to four protein subfamilies of TIFY, JAZ, ZML and PPD, and distributed unevenly on eight chromosomes. According to evolutionary relationship and structural characteristics, TIFY gene could be divided into seven groups, and members of each group had similar gene structure and conserved motif. (2)The promoter region of the CsTIFYs gene contains a varieties of cis-acting elements in response to abiotic stress, the quantitative experiments proved that its family members were highly responsive to Methyl jasmonate, salt (20%), cold (4 ℃) and drought (20% polyethylene glycol 6000) treatments, and some genes were highly expressed during the development of roots and apical buds. Based on the above results, it was speculated that the TIFY gene family may play a role in C. sinensis hormone signal regulation, stress defense response and growth and development.
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