Subjects: Biology >> Botany submitted time 2022-09-08 Cooperative journals: 《广西植物》
Abstract： Pimpinella candolleana is known as Miao ethnic herbal medicine in Guizhou for the treatment of icteric hepatitis, acute cholecystitis and other diseases. To investigate the chemical constituents of P. candolleana and their anti-inflammatory activities, the chemical constituents from the 70% ethanol extract of P. candolleana were separated by silica gel, Sephadex LH-20, Toyopearl HW-40F, Toyopearl HW-40C, ODS and other column chromatography, and their structures were elucidated by extensive spectroscopic analysis such as nuclear magnetic resonance (NMR) and mass spectrum (MS). The inflammatory cell model, built by LPS-induced RAW264.7 macrophage cells, was used to evaluate the anti-inflammatory activity. The results were as follows: (1): Detailed phytochemical study on P. candolleana led to the isolation and identification of twenty compounds were including vanillin (1), sesamin (2), 2-methyl-2-hydroxy-5-methoxy berz (d) hydrofuran-3-one (3), procatechin (4), 1,5-dihydroxy-2,3-dimethoxyxanthone (5), isorhamnetin (6), kaempferol (7), 8-hydroxy-2-methylchromone (8), luteolin (9), quercetin (10), 1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-[(2'R)-2'-hydroxypalmitoylamino]-8-octadecene-1,3,4- triol (11), isorhamnetin-3-O-β-D-galactopyranoside (12), isoquercitrin (13), norswertianolin (14), luteolin-6-C-α-L-arabinoside (15), kaempferol-3-O-β-D-galactopyranoside (16), kaempferol-7-O- β-D-glucopyranoside (17), luteolin-7-O-β-D-glucopyranoside (18), isovitexin (19), rutin (20). Compounds 1, 3, 4, 6, 7, 10, 13, 16, 18, and 20 were obtained from this plant for the first time. (2) The anti-inflammatory results showed that compounds 2-10, 12, 18 and 19 could significantly inhibit the LPS-induced NO content in RAW264.7 cells (P<0.05, P<0.01), and the inhibition rates of compounds 4, 7, 10, and 18 at a concentration of 25 μmol·L-1 were 57.37%, 83.60%, 68.16%, 81.14%, respectively. Overall, this study enriches the chemical constituents of P. candolleana, and clarifies that flavonoids are the active ingredients in the course of anti-inflammatory, which may provide a theoretical basis for further research and exploitation of P. candolleana.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： To study the chemical constituents of China endemic plant Rubia oncotricha, the compounds were isolated and purified by silica gel, ODS, Sephadex LH-20, and their structures were elucidated by modern spectroscopic techniques. Fifteen anthraquinone compounds were isolated and identified from Rubia oncotricha, they are 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-α-rhamnosyl(1→2)-β-glucoside (1), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-α-rhamnosyl(1→2)-β-glucoside (2), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(3'-O-acetyl)-α-rhamnosyl (1→2)-β-glucoside (3), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-β-glucoside (4), 1,3,6-trihydroxy-2-hydroxymethyl-9,10-anthraquinone-3-O-(6'-O-acetyl)-β-D-glucopyranoside (5), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-β-D-glucopyranoside (6), physcion-8-O-β-D-glucopyranoside (7), emodin-8-O-β-D-glucopyranoside (8), digiferruginol-11-O-β-gentiobioside (9), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-β-D-xylopyranosyl-(1→2)-β-Dglucopyranoside (10), 6-hydroxyrubiadin (11), 1,2-dihydroxyanthraquinone (12), chrysophanol (13), 6-hydroxyxanthopurpurin (14), 1,3-dihydroxyanthraquinone (15). Compounds 7, 8, 14 are isolated from the genus of Rubia for the first time, compound 1~6, 9, 10, 12, 13 are isolated from Rubia oncotricha for the first time.
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