西北南中国海棘冠海星(Acanthaster planci)的群体遗传学分析

• 作者： 夏利栋 ^1,2,3 那广水 ^1,2,3 王沛政 ^1,2,3
• 作者单位：
  1.  崖州湾创新研究院

  2.  海南省近岸海洋生态环境过程与碳汇重点实验室

  3.  生态环境学院
• 提交时间：2023-06-05

摘要: 本研究以西北南中国海部分岛礁的棘冠海星地理群体为研究对象，基于两种分子标记（线粒体COI基因序列和线粒体控制区CoReg序列），初步研究了西北南中国海部分岛礁之间棘冠海星的遗传结构和遗传多样性，并对其格局成因进行了探讨。对6个地理群体（东岛-DD，鹿回头-LHT，浪花礁-LH，华光礁-HG，玉琢礁-YZ，银屿-YY）共81个个体DNA序列进行特异性扩增，得到了81条长度为622bp的COI基因序列片段和81条长度为583bp的CoReg序列片段。对两种线粒体序列标记的分析表明，棘冠海星地理群体内存在差异的多样性(COI:h:0.286-0.824, ：0.00119-0.00272, k:0.743-1.692; CoReg:h:0.286-0.846, ：0.00686-0.01457, k:4-13.79)，CoReg序列片段展示出更高的多样性，地理群体DD均表现出最高的多样性，地理群体间存在一定的遗传结构(COI: FST:-0.00394-0.40513; CoReg: FST: 0.11198-0.78571)。单倍型网络关系图和STRUCTURE曲线图显示西北南中国海的棘冠海星群体可能分为两个遗传谱系，AMOVA分析结果支持西北南中国海棘冠海星地理群体间存在较高水平的遗传交流。Migration预测了一条自西沙群岛的地理群体LH、地理群体YZ，向西北方向经地理群体YY流向地理群体LHT的基因流动路线。认为南中国海表层洋流为棘冠海星浮浪幼虫在地理群体间的扩散提供动力，进一步构建了西北南中国海棘冠海星基因流模式。

Abstract: We used COI marker and mitochondrial control region (CoReg) marker to determine genetic structure and genetic diversity of Crown-of-thorns starfish (Acanthaster planci) (Asteroidea, Spinulosa) in the northwest South China Sea, the geographical population of A. planci among six locations: including Bombay Reef - LH, Discovery Reef - HG, Vuladdore Reef - YZ, Observation Bank - YY, Luhuitou Peninsula - LHT, Lincoin Island DD, were preliminarily studied, and the causes are discussed. We amplified the DNA sequences of 81 individuals from six populations, 81 COI sequences with a length of 622bp and 81 CoReg sequences with a length of 583bp were obtained. The analysis of two mitochondrial markers showed that there is a diversity of differences within the geographic population (COI: h: 0.286-0.824, : 0.00119-0.00272, k: 0.743-1.692; CoReg: h: 0.286-0.846, : 0.00686-0.01457, k: 4-13.79), CoReg sequence fragments show higher diversity, geographic population DD shows the highest diversity, there is a certain genetic structure between geographic populations (COI: FST: -0.00394-0.40513; CoReg: FST: 0.11198-0.78571). The haplotype network diagram and STRUCTURE analysis show that the geographic populations of A. planci in the Northwest South China Sea may be divided into two genetic lineages, with the results of AMOVA analysis support a high level of genetic communication among the geographic populations of A. planci in the northwest South China Sea. Migration is used to predict a gene flow route from geographic population LH and YZ in the Xisha Islands to geographic population LHT through geographic population YY in the northwest direction. The surface currents in the South China Sea provids impetus for the inter-population dispersal of A. planci larvae, which further construct the gene flow model of A. planci in the northwest South China Sea.

• 来自： 夏利栋
• 分类： 地球科学 >> 海洋科学
• 投稿状态： 已在期刊出版
• 引用： ChinaXiv:202306.00046 (或此版本 ChinaXiv:202306.00046V1)
  DOI:10.12074/202306.00046V1
  CSTR:32003.36.ChinaXiv.202306.00046.V1
• 推荐引用方式： 夏利栋,那广水,王沛政.(2023).西北南中国海棘冠海星(Acanthaster planci)的群体遗传学分析.中国科学院科技论文预发布平台.doi:10.12074/202306.00046V1 (点此复制)