滇水金凤花距发育相关基因ABP 的克隆及表达分析 postprint

Abstract: The purpose of this study was to explore the structural and expression characteristics of ABP gene from Impatiens uliginosa. ABP gene related to spur development of I. uliginosa was cloned by using RT-PCR method, whose homology and phylogenetic analysis of protein sequence were analyzed by using DNAMAN and MEGA softwares. In addition, the spatiotemporal expression patterns of ABP gene were investigated by qRT-PCR method. The results were as follows: (1) ABP gene of I. uliginosa was successfully cloned, whose full-length cDNA sequences was 627 bp, encoding 208 aa, and named IuABP. The protein encoded by ABP gene had the typical structure of Cupin superfamily proteins. (2) According to the result of its homology analysis, it showed that the homology of ABP gene of I. uliginosa reached 71% with those of I. glandulifera, Rose chinensis and Manihot esculenta. Based on phylogenetic analysis, it was found that IuABP and Impatiens glandulifera clustered into a branch, indicating they were the most closely related. (3) In accordance with qRT-PCR analysis, it showed that the expression level of IuABP were expressed in both three stages and two different parts of spur development of I. uliginosa. With the development of spur, the expression level of IuABP in the blade had a tendency of declining at the beginning and rising up in late, and reached the highest in the blooming stage. However, the expression level decreased gradually in the spur cup. In addition, the expression level of IuABP was the highest in the blade in the blooming stage. These results provide a theoretical basis for further studies on the function and the expression regulation mechanism of IuABP gene in spur development.