Abstract:
In order to determine the optimum conditions and antioxidant activity of total alkaloids
from leaves Mahonia fortunei by macroporous resins, the best resins were selected by comparing the
static adsorption and desorption effects of six kinds of macroporous adsorption resins on total
alkaloids. The process conditions for the dynamic purification of total alkaloids were investigated,
and the anti-oxidation performance of total alkaloids before and after purification was evaluated by
DPPH method. The results were as follows: (1) AB-8 macroporous adsorption resins had the best
purification effects. The optimum process conditions were crude herbal dose of 50 mg·mL-1, sample
volume of 26 BV, sample flow rate of 2 BV·h-1, eluted successively with 3 BV water and 4 BV 50%
ethanol. Under these optimized conditons, the content of total alkaloids was increased from 13.33%
to 56.64%. （ 2 ） The total alkaloids had good scavenging effects on DPPH· before and after purification. The scavenging rate of DPPH· was Vc (IC50=10.39 μg·mL-1) > purified total alkaloids
(IC50=39.08 μg·mL-1) > crude total alkaloids (IC50=55.28 μg·mL-1). AB-8 macroporous adsorption
resin can effectively enrich the effective parts of total alkaloids from leaves of Mahonia fortunei, and
the total alkaloids have certain antioxidant activities.
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