婆婆纳抗黑色素瘤物质基础的初步研究 postprint

Abstract: To clarify the anti-melanoma active extract and material basis of Veronica didyma Tenore, CCK8 method was used to evaluate the inhibitory effects of four extracts (petroleum ether extract, ethyl acetate extract, n-butanol extract, water extract), ethanol extract and monomer compounds on the proliferation of melanoma cell lines (B16 and A375); The chemical constituents of the active fraction were systematically separated and purified by phytochemical techniques and methods. The results were as follows: (1) Ethyl acetate extract (PPNE) had better inhibitory effect on the proliferation of B16 and A375 cells than other samples, and IC50 = 0.177 mg·mL-1 (B16), IC50 = 2.826 mg·mL-1 (A375), respectively. Seven monomers were obtained from PPNE were p-hydroxybenzaldehyde (1), Picroside II (2), isoscutellarein 7 - O - (6''' - Oacetyl) - β - allopyranosyl (1'''→2'') - β - glucopyranoside (3), 3' - hydroxyl - 4' - O - methylisoscutellarein 7 - O - [6''' - O - acetyl - β - D - allopyranosyl - (1→2) - β - D - Glucopyranoside (4), 6 - O - veratroylcatalposide (5), Veronicoside (6), isoscutellarein 4' - methyl ether 7 - O - (6''' - O - acetyl) - ballopyranosyl (1'''→2'') - β - glucopyranoside (7). (2) Seven compounds were isolated from this plant for the first time, and HPLC showed that these seven compounds are the major components of PPNE. Except Compound 1, the other six monomers all have good inhibitory effect on the proliferation of melanoma cells, and the anti-melanoma activities of compounds 3, 4 and 7 are reported for the first time. Based on these results, PPNE of Veronica didyma Tenore is an anti melanoma active fraction, Iridoids (compounds 2, 5, 6) and flavonoids (compounds 3, 4, 7) may be the basis of anti melanoma activity of PPNE. The results of this study will lay scientific foundation for the rational use of Veronica didyma Tenore sresources.