石韦不同极性萃取物体外降血糖活性研究 postprint

Abstract: In order to provide scientific basis for the further development and utilization of Pyrrosia lingua, the α-glucosidase and α-amylase inhibitory activities of different polar extracts of 95% ethanol extracts of Pyrrosia lingua were investigated by using p-nitrophenyl-α-D-glucopyranoside (pNPG) and DNS (3,5-dinitro salicylic acid (DNS) methods with acarbose as positive control. Enzyme kinetics method and Lineweaver-Burk curve were used to analyze the types of inhibition of α-glucosidase for the most active extract. The results showed that the IC50 values of the water extract were （4.71 ± 0.72）μg·mL-1 for α-glucosidase and （48.40 ± 0.32）μg·mL-1 for α-amylase respectively, which were significantly stronger than those of the other extracts (P < 0.05). The inhibition of α-glucosidase was stronger than that of acarbose, while the inhibition of α-amylase was weaker than that of acarbose. The IC50 values of acarbose on α-glucosidase and α-amylase were （2 857.36 ± 1.35） μg·mL-1 and （16.41 ± 0.63）μg·mL-1, respectively. The enzymatic kinetics showed that the inhibitory type on α-glucosidase of water extract was reversible inhibition, while the Lineweaver-Burk curve showed a competitive inhibition. In conclusion, the water extract from Pyrrosia lingua displayed potential inhibitory activity on α-glucosidase and α-amylase, which could be the natural sources of α-glucosidase inhibitor with less gastrointestinal side effects.