烟草种子成熟过程中膜脂的变化规律研究 postprint

Abstract: In order to clarify the change rule of membrane lipids in tobacco seed during ripening process,the changes rule of membrane Lipids content and components in the seeds of tobacco were studied systematically by the method of lipidomics. The results showed that the content of plastidic membrane lipid and its proportion in total membrane lipids decreased continuously with the ripening of the seeds, which were important lipids that constituted chloroplast and thylakoid membranes. Whereas as important lipids that constituting cell membranes, the content of extraplastidic membrane lipids decreased significantly at the early stage of seed maturation which reached a stable state at 21 days after pollination. The changes of total membrane Lipids content were similar to that of plastid membrane lipids, which reached a stable state at 29 days after pollination. Because grease accumulatedcontinuously during seed maturation, and its chemical structure was similar to that of membrane lipids, the reduction of plastidic membrane lipids content could be related to the sustained demand for greaseaccumulation during seed maturation and the decrease of demand for chloroplasts and thylakoids. The reason of extraplastidic membrane lipids remaining unchanged at 21 days after pollination was related to extraplastidic membrane lipids as the major membrane lipids involving in cell membrane, cell membrane played an important role during seed maturation and mature seed germination, therefore it was speculated that the decreased membrane lipids were transferred to grease.