分类： 生物学 >> 生理学 提交时间： 2019-03-28 合作期刊: 《干旱区科学》
摘要： Although exogenous application of glycinebetaine (GB) is widely reported to regulate a myriad of physio-biochemical attributes in plants under stressful environments including drought stress, there is little information available in the literature on how and up to what extent GB can induce changes in anatomical features in water starved plants. Thus, the present research work was conducted to assess the GB-induced changes in growth, physio-biochemical, and anatomical characteristics in two cultivars (CK-1 and F-411) of oat (Avena sativa L.) under limited water supply. After exposure to water stress, a considerable reduction was observed in plant growth in terms of lengths and weights of shoot and roots, leaf mesophyll thickness, leaf midrib thickness, root cortex thickness, root diameter, stem diameter, stem phloem area, and stem vascular bundle area in both oat cultivars. However, water stress resulted in a significant increase in leaf total phenolics, hydrogen peroxide (H2O2), ascorbic acid (AsA), GB contents, activities of enzymes (CAT, SOD and POD), total soluble proteins, leaf epidermis (abaxial and adaxial) thickness, bulliform cell area, sclerenchyma thickness, root endodermis and epidermis thickness, root metaxylem area, stem metaxylem area and stem sclerenchyma thickness in both oat cultivars. Foliar-applied 100 mM GB suppressed H2O2 contents, while improved growth attributes, free proline and GB contents, activity of SOD enzyme, leaf abaxial epidermis thickness, leaf bulliform cell area, leaf midrib thickness, leaf sclerenchyma thickness, root cortex thickness, root endodermis, epidermis thickness, root stele diameter, stem diameter, stem epidermis thickness, stem metaxylem area, and stem phloem and vascular bundle area in both oat cultivars. For both oat cultivars, CK-1 was superior to F-411 in leaf abaxial epidermis thickness, leaf mesophyll, leaf sclerenchyma, root metaxylem area, stem diameter, stem epidermis, sclerenchyma thickness, stem metaxylem area, and stem vascular bundle area. Overall, both oat cultivars showed inconsistent behavior to water stress and foliar-applied GB in terms of different physio-biochemical attributes, however, CK-1 was superior to F-411 in a number of anatomical features of leaf, root, and stem.
分类： 生物学 >> 生理学 提交时间： 2018-05-18 合作期刊: 《广西植物》
摘要： 建立一套在组织培养室内诱导试管薯的方法，获得直接下田的马铃薯种子，以弥补夏季由于高温而不能生产原原种的不足，扩充原原种的数量。论文以马铃薯米拉（Mira）品种的脱毒试管苗为试验材料，采用固液双层的培养方式，通过正交试验对米拉试管苗壮苗生长阶段和试管薯诱导阶段的培养基进行了优化；同时通过单因素试验，研究了蔗糖浓度、光照条件和CCC浓度对试管薯结薯的影响。结果表明：在固液双层培养中，米拉壮苗培养阶段优化的培养基为：改良MS培养基（MS培养基中硝酸铵变为2 000 mgL-1、硝酸钾变为2 000 mgL-1）+ 500 mgL-1 CCC + 0.1% 活性炭 + 0.1 mgL-1 DA-6 + 1 mgL-1 6-BA + 0.1 mgL-1 NAA + 3%蔗糖 + 6 gL-1 琼脂，PH 5.8；试管薯诱导及生长阶段优化的培养基为：MS1培养基（微量元素和铁盐的用量为MS培养基的2倍）+ 1.5% 活性炭+ 4 mgL-1 6-BA + 8% 蔗糖；在试管薯诱导阶段，弱光4 hd-1培养诱导的试管薯，在结薯指数、大薯率、薯块重量均优于暗培养。
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