Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise andcorrelation

分类： 物理学 >> 交叉学科物理及相关领域的科学与技术 提交时间： 2016-05-08

rui li liufang xu hualinshi

摘要： Genes are organized into operons in procaryote, and these genes in one operon generally have related functions. However, genes in the same operon are usually not equally expressed, and the ratio needs to be fine-tuned for specific functions. We examine the difference of gene expression noise and correlationwhen tuning the expression level at the transcriptional or translational level in a bicistronic operon driven by a constitutive or a two-state promoter. We get analytic results for the noise and correlation of gene expression levels, which is confirmed by our stochastic simulations. Both the noise and the correlation ofgene expressions in an operon with a two-state promoter are higher than in an operon with a constitutive promoter. Premature termination of mRNA induced by transcription terminator in the intergenic region or changing translation rates can tune the protein ratio at the transcriptional level or at the translational level. We find that gene expression correlation between promoter-proximal and promoter-distal genes at the protein level decreases as termination increases. Incontrast, changing translation rates in the normal range almost does not alter the correlation. This explains why the translation rate is a key factor ofmodulating gene expressions in an operon. Our results can be useful to understand the relationship between the operon structure and the biological function ofa gene network, and also may help in synthetic biology design.

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