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1. chinaXiv:201804.01934 [pdf]


Wang, Xin
Subjects: Biology >> Biological Evolution


submitted time 2018-04-20 Hits1715Downloads706 Comment 0

2. chinaXiv:201605.01576 [pdf]


Zhang, Lei; He, Jiansen; Tu, Chuanyi; Yang, Liping; Wang, Xin; Marsch, Eckart; Wang, Linghua
Subjects: Geosciences >> Space Physics

MHD discontinuities are ubiquitous in the solar wind and are often found at the origin of turbulence intermittency. They may also play a key role in the turbulence dissipation and heating of the solar wind. The tangential discontinuities (TDs) and rotational discontinuities (RDs) are the two most important types of discontinuities. Recently, the connection between turbulence intermittency and proton thermodynamics has been observationally investigated. Here, we present numerical results from a three-dimensional MHD simulation with pressure anisotropy and we define new methods for identifying and distinguishing TDs and RDs. Three statistical results obtained for the relative occurrence rates and heating effects are highlighted: (1) RDs tend to take up the majority of the discontinuities along with time;(2) the thermal states embedding TDs tend to be associated with extreme plasma parameters or instabilities while RDs do not;(3) TDs have a higher average T as well as perpendicular temperature T.. The simulation shows that TDs and RDs evolve and contribute to solar wind heating differently. These results will improve our understanding of the mechanisms that generate discontinuities and cause plasma heating.

submitted time 2016-05-12 Hits1010Downloads630 Comment 0

3. chinaXiv:201605.01570 [pdf]


Yang, Liping; Zhang, Lei; He, Jiansen; Tu, Chuanyi; Wang, Linghua; Marsch, Eckart; Wang, Xin; Zhang, Shaohua; Feng, Xueshang
Subjects: Geosciences >> Space Physics

Measurements of solar wind turbulence reveal the ubiquity of discontinuities. In this study we investigate how the discontinuities, especially rotational discontinuities (RDs), are formed in MHD turbulence. In a simulation of the decaying compressive three-dimensional (3D) MHD turbulence with an imposed uniform background magnetic field, we detect RDs with sharp field rotations and little variations of magnetic field intensity, as well as mass density. At the same time, in the de Hoffman-Teller frame, the plasma velocity is nearly in agreement with the Alfven speed, and is field-aligned on both sides of the discontinuity. We take one of the identified RDs to analyze its 3D structure and temporal evolution in detail. By checking the magnetic field and plasma parameters, we find that the identified RD evolves from the steepening of the Alfven wave with moderate amplitude, and that steepening is caused by the nonuniformity of the Alfven speed in the ambient turbulence.

submitted time 2016-05-12 Hits1053Downloads563 Comment 0

4. chinaXiv:201605.00730 [pdf]

Crystal Structure of the Core Region of Hantavirus Nucleocapsid Protein Reveals the Mechanism for Ribonucleoprotein Complex Formation

Guo, Yu; Ma, Chao; Wang, Xu; Wang, Xin; Liu, Pi; Lin, Jianping; Guo, Yu; Ma, Chao; Wang, Xu; Wang, Xin; Liu, Pi; Lin, Jianping; Lou, Zhiyong; Lou, Zhiyong; Sun, Yuna; Lou, Zhiyong; Shen, Shu; Deng, Fei; Wang, Hualin; Wang, Wenming
Subjects: Biology >> Biophysics

Hantaviruses, which belong to the genus Hantavirus in the family Bunyaviridae, infect mammals, including humans, causing either hemorrhagic fever with renal syndrome (HFRS) or hantavirus cardiopulmonary syndrome (HCPS) in humans with high mortality. Hantavirus encodes a nucleocapsid protein (NP) to encapsidate the genome and form a ribonucleoprotein complex (RNP) together with viral polymerase. Here, we report the crystal structure of the core domains of NP (NPcore) encoded by Sin Nombre virus (SNV) and Andes virus (ANDV), which are two representative members that cause HCPS in the New World. The constructs of SNV and ANDV NPcore exclude the N- and C-terminal portions of full polypeptide to obtain stable proteins for crystallographic study. The structure features an N lobe and a C lobe to clamp RNA-binding crevice and exhibits two protruding extensions in both lobes. The positively charged residues located in the RNA-binding crevice play a key role in RNA binding and virus replication. We further demonstrated that the C-terminal helix and the linker region connecting the N-terminal coiled-coil domain and NPcore are essential for hantavirus NP oligomerization through contacts made with two adjacent protomers. Moreover, electron microscopy (EM) visualization of native RNPs extracted from the virions revealed that a monomer-sized NP-RNA complex is the building block of viral RNP. This work provides insight into the formation of hantavirus RNP and provides an understanding of the evolutionary connections that exist among bunyaviruses. IMPORTANCE Hantaviruses are distributed across a wide and increasing range of host reservoirs throughout the world. In particular, hantaviruses can be transmitted via aerosols of rodent excreta to humans or from human to human and cause HFRS and HCPS, with mortalities of 15% and 50%, respectively. Hantavirus is therefore listed as a category C pathogen. Hantavirus encodes an NP that plays essential roles both in RNP formation and in multiple biological functions. NP is also the exclusiv

submitted time 2016-05-05 Hits1119Downloads581 Comment 0

5. chinaXiv:201605.00708 [pdf]

Yersinia spp. Identification Using Copy Diversity in the Chromosomal 16S rRNA Gene Sequence

Hao, Huijing; Liang, Junrong; Duan, Ran; Chen, Yuhuang; Liu, Chang; Xiao, Yuchun; Li, Xu; Jing, Huaiqi; Wang, Xin; Liu, Chang; Su, Mingming;
Subjects: Biology >> Biophysics

API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g. Yersinia ferderiksenii and Yersinia intermedia, which need a variety of molecular biology methods as auxiliaries for identification. The 16S rRNA gene is considered a valuable tool for assigning bacterial strains to species. However, the resolution of the 16S rRNA gene may be insufficient for discrimination because of the high similarity of sequences between some species and heterogeneity within copies at the intra-genomic level. In this study, for each strain we randomly selected five 16S rRNA gene clones from 768 Yersinia strains, and collected 3,840 sequences of the 16S rRNA gene from 10 species, which were divided into 439 patterns. The similarity among the five clones of 16S rRNA gene is over 99% for most strains. Identical sequences were found in strains of different species. A phylogenetic tree was constructed using the five 16S rRNA gene sequences for each strain where the phylogenetic classifications are consistent with biochemical tests; and species that are difficult to identify by biochemical phenotype can be differentiated. Most Yersinia strains form distinct groups within each species. However Yersinia kristensenii, a heterogeneous species, clusters with some Yersinia enterocolitica and Yersinia ferderiksenii/intermedia strains, while not affecting the overall efficiency of this species classification. In conclusion, through analysis derived from integrated information from multiple 16S rRNA gene sequences, the discrimination ability of Yersinia species is improved using our method.

submitted time 2016-05-05 Hits939Downloads483 Comment 0

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