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1. chinaXiv:202102.00028 [pdf]

Taurine is found in rumen fluid, plasma and urine of beef cattle fed a ration containing sodium sulfate

Zhao, Yuchao ; Zhao, Guangyong
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Animal Husbandry and Veterinary Medicine

Objective: The objectives of the experiment were to study the effects of dietary supplementation with sodium sulfate (Na2SO4) on taurine concentrations of plasma, urine and rumen fluid in steers. Methods: Six Simmental steers (bodyweight 449 ± 19 kg) were used as experimental animals. Three levels of Na2SO4, i.e. 0, 20 and 40 g/d, were added in a basal ration as experimental treatments. The animals and the dietary treatments were randomly allocated in a replicated 3 × 3 Latin square design. Each experimental period included 15 days for adaptation and 5 days for sampling. Rumen fluid, blood and urine were sampled from the steers during each sampling period. Results: Dietary addition with Na2SO4 at 40 g/d increased plasma taurine concentration (P < 0.05) and tended to increase the plasma taurine concentration with increasing Na2SO4 level in a linear manner (P = 0.052). A linear positive regression relationship was found between plasma taurine concentration (?g/mL) and sulfur intake (g/d) (R2 = 0.18, P = 0.046). Taurine was found in rumen fluid and urine. No differences were found in ruminal taurine concentrations or urinary taurine excretions among treatments (P > 0.05). Limitations: We infer that the taurine in rumen fluid should have come from the blood through saliva secretion or/and the rumen microbial synthesis. However, no reports are available on the hypothesis. Further research is needed to investigate the possible origins of ruminal taurine as well as the impacts of taurine on rumen microecosystem and fermentation. Conclusions: Taurine has been found in rumen fluid for the first time in the experiment. Dietary addition with Na2SO4 increased plasma taurine concentrations whereas it did not affect urinary taurine excretions in steers.

submitted time 2021-02-08 Hits1258Downloads635 Comment 0

2. chinaXiv:201605.01181 [pdf]

Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts

Zhao, Jiating; Gao, Yuxi; Peng, Xiaomin; Li, Yunyun; Xu, Xiaohan; Li, Bai; Dong, Juncai; Li, Yu-Feng; Pu, Yunxia; Wu, Gang; Zhu, Nali
Subjects: Biology >> Biophysics

A comprehensive approach that can identify and quantify selenium (Se) in seleno-proteins in Se-enriched yeast was developed. The Se-containing compounds in Se-enriched yeast were first extracted and then the fraction of Se-containing proteins in the supernatant was analyzed by 2-dimensional electrophoresis (2-DE) and synchrotron radiation X-ray fluorescence (SR-XRF). The detection limit (DL) of SR-XRF analysis for Se quantification in Se-containing proteins after 2-DE separation was calculated to be 0.20 mu g g(-1), which is suitable for Se quantification in the Se-containing spots present on the 2-D gel. After being scanned by SR-XRF, only spots with a mean Se content exceeding twice the DL of SR-XRF were considered to be seleno-proteins. In this way, a total of 157 Se-containing spots in the gel were visually distinguished. Se contents in all the Se-containing proteins of different molecular weights were quantified. The total Se content on the 2-D gel was calculated to be 126.56 mu g g(-1), which covered most of the seleno-proteins on the 2-D gel.

submitted time 2016-05-11 Hits1231Downloads717 Comment 0

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