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1. chinaXiv:201711.00276 [pdf]

DPABI: Data Processing & Analysis for (Resting-State) Brain Imaging

Chao-Gan Yan; Xin-Di Wang; Xi-Nian Zuo; Yu-Feng Zang
Subjects: Psychology >> Applied Psychology

Brain imaging efforts are being increasingly devoted to decode the functioning of the human brain. Among neuroimaging techniques, resting-state fMRI (R-fMRI) is currently expanding exponentially. Beyond the general neuroimaging analysis packages (e.g., SPM, AFNI and FSL), REST and DPARSF were developed to meet the increasing need of user-friendly toolboxes for R-fMRI data processing. To address recently identified methodological challenges of R-fMRI, we introduce the newly developed toolbox, DPABI, which was evolved from REST and DPARSF. DPABI incorporates recent research advances on head motion control and measurement standardization, thus allowing users to evaluate results using stringent control strategies. DPABI also emphasizes test-retest reliability and quality control of data processing. Furthermore, DPABI provides a user-friendly pipeline analysis toolkit for rat/monkey R-fMRI data analysis to reflect the rapid advances in animal imaging. In addition, DPABI includes preprocessing modules for task-based fMRI, voxel-based morphometry analysis, statistical analysis and results viewing. DPABI is designed to make data analysis require fewer manual operations, be less time-consuming, have a lower skill requirement, a smaller risk of inadvertent mistakes, and be more comparable across studies. We anticipate this open-source toolbox will assist novices and expert users alike and continue to support advancing R-fMRI methodology and its application to clinical translational studies.

submitted time 2017-11-06 Hits7966Downloads2212 Comment 0

2. chinaXiv:201605.01193 [pdf]

HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions

Zhang, Yanqing; Cao, Wei; Li, Ningning; Zhang, Yiudao; Zhang, Xiaoxiao; Lei, Jianlin; Gao, Ning; Mandava, Chandra Sekhar; Sanyal, Suparna; Li, Xiaojing; Mi, Kaixia; Zhang, Dejiu; Qin, Yan
Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology

Adverse cellular conditions often lead to nonproductive translational stalling and arrest of ribosomes on mRNAs. Here, we used fast kinetics and cryo-EM to characterize Escherichia coil HflX, a GTPase with unknown function. Our data reveal that HflX is a heat shock-induced ribosome-splitting factor capable of dissociating vacant as well as mRNA-associated ribosomes with deacylated tRNA in the peptidyl site. Structural data demonstrate that the N-terminal effector domain of HflX binds to the peptidyl transferase center in a strikingly similar manner as that of the class I release factors and induces dramatic conformational changes in central intersubunit bridges, thus promoting subunit dissociation. Accordingly, loss of HflX results in an increase in stalled ribosomes upon heat shock, These results suggest a primary role of HflX in rescuing translationally arrested ribosomes under stress conditions.

submitted time 2016-05-11 Hits532Downloads331 Comment 0

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