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1. chinaXiv:201605.01430 [pdf]

CUG-BP1 regulates RyR1 ASI alternative splicing in skeletal muscle atrophy

Tang, Yinglong; Wang, Huiwen; Gu, Lei; Yang, Zhiguang; Wu, Yanyun; Yuan, Qi; Ji, Guangju; Wei, Bin; Tang, Yinglong; Guo, Yuting; Gu, Lei; Yang, Zhiguang; Zhao, Gang; Zhang, Qing
Subjects: Biology >> Biophysics

RNA binding protein is identified as an important mediator of aberrant alternative splicing in muscle atrophy. The altered splicing of calcium channels, such as ryanodine receptors (RyRs), plays an important role in impaired excitation-contraction (E-C) coupling in muscle atrophy; however, the regulatory mechanisms of ryanodine receptor 1 (RyR1) alternative splicing leading to skeletal muscle atrophy remains to be investigated. In this study we demonstrated that CUG binding protein 1 (CUG-BP1) was up-regulated and the alternative splicing of RyR1 ASI (exon70) was aberrant during the process of neurogenic muscle atrophy both in human patients and mouse models. The gain and loss of function experiments in vivo demonstrated that altered splicing pattern of RyR1 ASI was directly mediated by an up-regulated CUG-BP1 function. Furthermore, we found that CUG-BP1 affected the calcium release activity in single myofibers and the extent of atrophy was significantly reduced upon gene silencing of CUG-BP1 in atrophic muscle. These findings improve our understanding of calcium signaling related biological function of CUG-BP1 in muscle atrophy. Thus, we provide an intriguing perspective of involvement of mis-regulated RyR1 splicing in muscular disease.

submitted time 2016-05-12 Hits627Downloads379 Comment 0

2. chinaXiv:201605.01392 [pdf]

Proteomic Comparison and MRM-Based Comparative Analysis of Metabolites Reveal Metabolic Shift in Human Prostate Cancer Cell Lines

Shu, Qingbo; Cai, Tanxi; Chen, Xiulan; Xue, Peng; Zhu, Nali; Xie, Zhensheng; Wei, Shasha; Niu, Lili; Yang, Fuquan; Shu, Qingbo; Cai, Tanxi; Chen, Xiulan; Xue, Peng; Zhu, Nali; Xie, Zhensheng; Wei, Shasha; Niu, Lili; Yang, Fuquan; Shu, Qingbo; Zhang, Qing
Subjects: Biology >> Biophysics

One of the major challenges in prostate cancer therapy remains the development of effective treatments for castration-resistant prostate cancer (CRPC), as the underlying mechanisms for its progression remain elusive. Previous studies showed that androgen receptor (AR) is crucially involved in regulation of metabolism in prostate cancer (PCa) cells throughout the transition from early stage, androgen-sensitive PCa to androgen-independent CRPC. AR achieves such metabolic rewiring directively either via its transcriptional activity or via interactions with AMP-activated protein kinase (AMPK). However, due to the heterogeneous expression and activity status of AR in PCa cells, it remains a challenge to investigate the links between AR status and metabolic alterations. To this end, we compared the proteomes of three pairs of androgen-sensitive (AS) and androgen-independent (AI) PCa cell lines, namely, PC3-AR(+)/PC3, 22Rv1/Du145, and LNCaP/C42B, using an iTRAQ labeling approach. Our results revealed that most of the differentially expressed proteins between each pair function in metabolism, indicating a metabolic shift between AS and AT cells, as further validated by multiple reaction monitoring (MRM)-based quantification of nucleotides and relative comparison of fatty acids between these cell lines. Furthermore, increased adenylate kinase isoenzyme 1 (AK1) in AS relative to AT cells may result in activation of AMPK, representing a major regulatory factor involved in the observed metabolic shift in PCa cells.

submitted time 2016-05-12 Hits767Downloads450 Comment 0

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