Current Location:home > Browse

Submitted Date

1. chinaXiv:202003.00008 [pdf]

Germline Polymorphisms and Length of Survival of Nasopharyngeal Carcinoma: An Exome-Wide Association Study in Multiple Cohorts

Yun-Miao Guo; Jie-Rong Chen; Yan-Chun Feng; Chua, Melvin L. K. ; Zeng, Yanni ; Edwin Pun Hui; Allen K. C. Chan; Lin-Quan Tang; Lin Wang; Qian Cui; Hui-Qiong Han; Chun-Ling Luo; Guo-Wang Lin; Yan Liang; Yang Liu; Zhong-Lian He; Yu-Xiang Liu; Pan-Pan Wei; Chu-Jun Liu; Wan Peng
Subjects: Medicine, Pharmacy >> Clinical Medicine

Germline polymorphisms have been linked with differential survival outcomes in cancers but have not been well studied in nasopharyngeal carcinoma (NPC). Here, two-phase association study is conducted to discover germline polymorphisms that are associated with the prognosis of NPC. The discovery phase includes two consecutive hospital cohorts of patients with NPC from Southern China. Exome-wide genotypes at 246,173 single nucleotide polymorphisms (SNPs) are determined, followed by survival analysis for each SNP under Cox proportional hazards regression model. Candidate SNP is replicated in another two independent cohorts from Southern China and Singapore. Meta-analysis of all samples (n = 5,553) confirm that the presence of rs1131636-T, located in the 3′-UTR of RPA1, confers an inferior overall survival (HR = 1.33, 95% CI = 1.20-1.47, P = 6.31 × 10-8). Bioinformatics and biological assays show that rs1131636 has regulatory effects on upstream RPA1. Functional studies further demonstrate that RPA1 promoted the growth, invasion, migration, and radioresistance of NPC cells. Additionally, miR-1253 has been identified as a suppressor for RPA1 expression, likely through regulation of its binding affinity to rs1131636 locus. Collectively, these findings provide a promising biomarker aiding in stratifying patients with poor survival, as well as a potential drug target for NPC.

submitted time 2020-03-06 Hits7891Downloads272 Comment 0

2. chinaXiv:201705.00596 [pdf]

Modeling of Torque Output and Magnetic Force for Novel Spherical Actuator with Three-dimensional Pole Arrays

Yan, L [Yan, Liang][ 1,2 ]; Liang, FQ [Liang, Fengqi][ 1 ]; Wu, ZW [Wu, Zewu][ 1 ]; Jiao, ZX [Jiao, Zongxia][ 1 ]; Chen, CY [Chen, Chin-Yin][ 3 ]; Chen, IM [Chen, I-Ming][ 4 ]
Subjects: Physics >> General Physics: Statistical and Quantum Mechanics, Quantum Information, etc.

A novel PM spherical actuator based on three- dimensional pole array is proposed and developed in this paper. Conventionally, 2D pole arrays are widely employed in the design of spherical actuators, which constraints the system torque output greatly. The concept of 3D pole array is aimed at improving the torque performance. The torque has been analyzed and the corresponding analytical model is established based on curve fitting method (CFM) due to the importantance to real-time control. Magnetic force has been studied in a similar way. The results shows that the modeling method has a relatively high precision and can be further used in the real-time control.

submitted time 2017-05-02 Hits9535Downloads226 Comment 0

3. chinaXiv:201605.00404 [pdf]

The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein

Wenming Zhao; Chunmei Guan; Jian Feng; Yan Liang; Ni Zhan; Jianru Zuo; Bo Ren
Subjects: Biology >> Botany >> Plant physiology

In Arabidopsis, the phytohormone abscisic acid (ABA) plays a vital role in inhibiting seed germination and in post-germination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1, and was degraded through the 26S proteasome pathway. However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI (CRWN) proteins, predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-controlled seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type. Genetic analysis suggested that CRWNs act upstream of ABI5. The observation that CRWN3 colocalizes with ABI5 in nuclear bodies indicates that CRWNs might participate in ABI5 protein degradation in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination.

submitted time 2016-05-03 Hits1073Downloads286 Comment 0

  [1 Pages/ 3 Totals]