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1. chinaXiv:202002.00014 [pdf]

Clinical and Biochemical Indexes from 2019-nCoV infected patients linked to viral loads and lung injury

Yingxia Liu; Yang Yang; Cong Zhang; Fengming Huang; Fuxiang Wang; Jing Yuan; Zhaoqin Wang; Jingxiu Li; Jianming Li; Cheng Feng; Zheng Zhang; Lifei Wang; Ling Peng; Li Chen; Yuhao Qin; Dandan Zhao; Shuguang Tan; Lu Yin; Jun Xu; Congzhao Zhou
Subjects: Biology >> Biochemistry

新型冠状病毒(2019-nCoV)自2019年12月在湖北省武汉市爆发,并迅速传播到中国多地及其他国家。在本研究中,我们报告了来自中国深圳早期的2019-nCoV感染患者的流行病学、临床指标、生化指标和影像学特征,以及可用于预测疾病严重程度的潜在生物标记物。所有12例2019-nCoV感染的肺炎患者均发展为肺炎,其中一半患者进一步发展为急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)。最常见的实验室检测生化指标异常是低白蛋白(albumine,ALB)血症、淋巴细胞(lymphocytes,LYM)计数减少,淋巴细胞百分比和中性粒细胞(neutrophils,NEU)百分比降低,C反应蛋白(C-reactive protein,CRP)和乳酸脱氢酶(lactate dehydrogenase,LDH)水平升高,以及CD8细胞计数降低。从患者呼吸道特别是下呼吸道检测到的2019-nCoV病毒滴度与肺部疾病的严重程度正相关。 ALB、LYM、LYM(%)、LDH、NEU(%)和CRP的水平与急性肺损伤程度高度相关。年龄、病毒滴度、肺损伤评分和血液生化指标:ALB、CRP、LDH、LYM(%)、LYM和NEU(%)可能是疾病严重程度的预测指标。此外, 2019-nCoV感染患者的血浆血管紧张素II水平显着升高,并且与病毒滴度和肺损伤程度线性相关。我们的研究结果提供了多种潜在的可用于诊断的生物标志物, 并提出了血管紧张素 II受体阻滞剂(angiotensin II receptor blocker,ARB)药物或可作为治疗2019-nCoV感染的潜在药物进行深入研究。

submitted time 2020-02-08 Hits7258Downloads763 Comment 0

2. chinaXiv:201605.01355 [pdf]

KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum

Li, Ming Yuan; Kwok, Kevin; Siu, Yu Lam; Zhang, Jing Shu; Kudelko, Mateusz; Bruzzone, Roberto; Wang, Pei Gang; Li, Ming Yuan; Kwok, Kevin; Siu, Yu Lam; Zhang, Jing Shu; Kudelko, Mateusz; Bruzzone, Roberto; Wang, Pei Gang; Grandadam, Marc; Sayteng, Kouxiong; Lagache, Thibault; Olivo-Marin, Jean-Christophe; Lagache, Thibault; Olivo-Marin, Jean-Christophe
Subjects: Biology >> Biophysics >> Cell Biology

Membrane receptors at the surface of target cells are key host factors for virion entry; however, it is unknown whether trafficking and secretion of progeny virus requires host intracellular receptors. In this study, we demonstrate that dengue virus (DENV) interacts with KDEL receptors (KDELR), which cycle between the ER and Golgi apparatus, for vesicular transport from ER to Golgi. Depletion of KDELR by siRNA reduced egress of both DENV progeny and recombinant subviral particles (RSPs). Coimmunoprecipitation of KDELR with dengue structural protein prM required three positively charged residues at the N terminus, whose mutation disrupted protein interaction and inhibited RSP transport from the ER to the Golgi. Finally, siRNA depletion of class II Arfs, which results in KDELR accumulation in the Golgi, phenocopied results obtained with mutagenized prME and KDELR knockdown. Our results have uncovered a function for KDELR as an internal receptor involved in DENV trafficking.

submitted time 2016-05-11 Hits363Downloads218 Comment 0

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