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基于mRNA 5’端TIR区二级结构优化提高重组sTNFα RI在大肠杆菌中的表达水平 Biology 2018-01-16

秦娇荣 成都生物制品研究所有限责任公司, 成都 610023 ; 赵兆 成都生物制品研究所有限责任公司, 成都 610023 ; 罗心梅 成都生物制品研究所有限责任公司, 成都 610023 ; 李春阳 成都生物制品研究所有限责任公司, 成都 610023 ;
目的:通过优化PET11b-sTNFαRI 5’ mRNA翻译起始区(TIR)二级结构从而提高可溶性肿瘤坏死因子I型受体(sTNFαRI)在大肠杆菌(E.coli BL21(DE3))中的表达水平。方法:通过对PET11b-sTNFa RI mRNA 5’端TIR区二级结构的自由能及核苷酸位置熵分...

Article Totals:9,604

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