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Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors

Abstracts

Wilms' tumors are one of the most common malignant, solid intra-abdominal tumors observed in children. Although potential tumor markers have been found, inflammatory cytokines interfere with the process of specific protein identification. The present study was undertaken to identify post-traumatic stress-related factors of Wilms' tumors and to verify the accuracy of early-stage tumor-specific serum protein markers. Serum samples were screened for differentially-expressed proteins using surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS). Potential markers were isolated and purified using solid-phase extraction (SPE) and SDS-PAGE. Following enzymatic digestion of the protein samples, the peptide fragments were detected with high performance liquid chromatography-mass spectrometry. The obtained peptide mass fingerprint was searched in the Swiss-Prot protein sequence database via the Mascot search engine. Differentially-expressed proteins were verified using western blot analysis. Differentially-expressed proteins with a mass/charge of 5,816 were screened out using SELDI-TOF-MS, and significant differences between the tumor and control groups, and the trauma and control groups were observed. Target proteins were isolated and purified using SPE and SDS-PAGE. Thioredoxin 1 (Trx1) was found to be differentially expressed. In the serum of children with Wilms' tumors, there was an increase in the level of the post-traumatic stress-related inflammatory factor, Trx1, as compared with the normal control group. Thus, the results of this study indicate that Trx1 presents a potential post-traumatic stress-related factor of Wilms' tumors.
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Journal:ONCOLOGY LETTERS
Recommended references: Zhang, Junjie,Guo, Fei,Wang, Lei,Zhao, Wei,Zhang, Da,Yang, Heying,Wang, Jiaxiang,Hu, Qian,Yu, Jiekai,Zheng, Shu,Niu, Lili,Yang, Fuquan,.(2016).Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors.ONCOLOGY LETTERS.[ChinaXiv:201605.00771] (Click&Copy)
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[V1] 2016-05-05 14:39:50 chinaXiv:201605.00771V1 Download
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